Anti-Caspase 3/CASP3 (p17) Antibody (Clone#AOE-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM3957

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of anti-Caspase 3/CASP3 antibody (BM3957). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 3/CASP3 antigen affinity purified monoclonal antibody (BM3957) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 3/CASP3 at approximately 32 kDa. The expected band size for Caspase 3/CASP3 is at 32 kDa.
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Figure 2. IHC analysis of Caspase 3/CASP3 using anti-Caspase 3/CASP3 antibody (BM3957) .
Caspase 3/CASP3 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Caspase 3/CASP3 Antibody (BM3957) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Figure 3. IHC analysis of Caspase 3/CASP3 using anti-Caspase 3/CASP3 antibody (BM3957) .
Caspase 3/CASP3 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Caspase 3/CASP3 Antibody (BM3957) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
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Immunofluorescent analysis of Hela cells, using Caspase-3 Antibody .
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Immunoprecipitate (IP) analysis using the Antibody. (wb)
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产品名称

Anti-Caspase 3/CASP3 (p17) Antibody (Clone#AOE-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apopain; CASP 3; CASP3; Caspase 3; Caspase 3/p17; Caspase3; CPP 32; CPP32; CPP32B; Cysteine protease CPP32; Protein Yama; SCA 1; SREBP cleavage activity 1

产品类型

Monoclonal

检验物种

human, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

CASP3

克隆号

AOE-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Caspase-3

计算分子量

32 kDa

实际分子量

35 kDa,(cleaved)20/17/12 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.

背景资料

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position.

Uniprot ID

P42574

文献引用格式

Caspase 3/CASP3 (p17) Antibody (Clone#AOE-3) (Boster Biological Technology, Wuhan, China. Catalog#BM3957)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

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