Anti-HSP70 Antibody (Clone#BRM-22)

Mouse monoclonal antibody

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筛选器： All WB IHC

BM0368

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- HSP70 antibody (BM0368). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: U20S whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse liver tissue lysates.
Use mouse anti- HSP70 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for HSP70 at approximately 70KD. The expected band size for HSP70 is at 70KD.
all(2)
IHC analysis using anti- HSP70 antibody (BM0368). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
all(2)

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产品名称

Anti-HSP70 Antibody (Clone#BRM-22)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

HSP70-1; HSP70.1; HSPA1A; HSP72; HSPA1; HSX70; HEL-S-103; HSP70; HSP70-1A; HSP70-2; HSP70.2; HSP70I; HSP72; HSPA1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

HSPA1A

克隆号

BRM-22

宿主

Mouse

抗体亚型

IgG1

免疫原

HSP70 isolated from bovine brain.

计算分子量

70 kDa

实际分子量

70 kDa

成分

200 ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA

纯化方式

Ascites

浓度

200ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex(MHC) class III region 92 kilobases(kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

Uniprot ID

P0DMV8

RRID

AB_3713608

文献引用格式

HSP70 Antibody (Clone#BRM-22) (Boster Biological Technology, Wuhan, China. Catalog#BM0368)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

heat shock protein family A (Hsp70) member 1A

蛋白名全称

Heat shock 70 kDa protein 1A

实验方案>

实验步骤

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