Anti-HSP70 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

PB9638

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HSP70 using anti-HSP70 antibody (PB9638). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human SW620 whole cell lysates,
Lane 6: human PANC-1 whole cell lysates,
Lane 7: human Raji whole cell lysates,
Lane 8: rat brain tissue lysates,
Lane 9: rat kidney tissue lysates,
Lane 10: mouse kidney tissue lysates,
Lane 11: mouse NIH/3T3 whole cell lysates,
Lane 12: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP70 antigen affinity purified polyclonal antibody (PB9638) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.
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IHC analysis of HSP70 using anti-HSP70 antibody (PB9638).
HSP70 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSP70 Antibody (PB9638) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of HSP70 using anti-HSP70 antibody (PB9638).
HSP70 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSP70 Antibody (PB9638) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of HSP70 using anti-HSP70 antibody (PB9638).
HSP70 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSP70 Antibody (PB9638) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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ICC/IF analysis of HSP70 using anti-HSP70 antibody (PB9638).
HSP70 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-HSP70 Antibody (PB9638) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of Hela cells using anti-HSP70 antibody (PB9638).
Overlay histogram showing Hela cells stained with PB9638 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSP70 Antibody (PB9638) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-HSP70 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

HSP70-1; HSP70.1; HSPA1A; HSP72; HSPA1; HSX70; HEL-S-103; HSP70; HSP70-1A; HSP70-2; HSP70.2; HSP70I; HSP72; HSPA1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

HSPA1A

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70, different from the related mouse sequence by five amino acids, and from the related rat sequence by three amino acids.

计算分子量

70 kDa

实际分子量

70 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

HSPA1 (heat shock 70kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al. (1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

Uniprot ID

P0DMV8

RRID

AB_3082197

文献引用格式

HSP70 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9638)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

heat shock protein family A (Hsp70) member 1A

蛋白名全称

Heat shock 70 kDa protein 1A

实验方案>

实验步骤

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