产品名称
Human TNFRSF5/CD40 ELISA Kit
指标别名
B cell surface antigen CD40; Bp50; CD40; CD40L receptor; CDW40; p50; TNFRSF5
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
CD40 molecule, TNF receptor superfamily member 5
文献引用格式
Human TNFRSF5/CD40 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0702)
背景资料
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0702-CAP | 96-well plate precoated with anti- human CD40 antibody | 1 |
| EK0702-ST | Lyophilized recombinant human CD40 standard | 10ng/tube×2 |
| EK0702-DA | Biotinylated anti- human CD40 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 32 | 167 | 443 | 32 | 177 | 404 |
| Standard deviation | 1.66 | 9.18 | 34.99 | 1.92 | 10.79 | 38.78 |
| CV(%) | 5.2 | 5.5 | 7.9 | 6 | 6.1 | 9.6 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.032 | 0.078 | 0.149 | 0.245 | 0.413 | 0.751 | 1.246 | 1.952 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk