产品名称
Mouse TNFRSF5/CD40 Quick ELISA Kit
指标别名
B cell surface antigen CD40; Bp50; CD40; CD40L receptor; CDW40; p50; TNFRSF5
免疫原
Expression system for standard: NS0; Immunogen sequence: V24-R193
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
CD40 molecule, TNF receptor superfamily member 5
文献引用格式
Mouse TNFRSF5/CD40 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0703)
背景资料
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
试剂盒内容
| Catalog number | Description | Quantity |
| FEK0663-CAP | 96-well plate precoated with anti-Mouse CD40 antibody | 1 |
| FEK0663-ST | lyophilized recombinant Mouse CD40 standard | 10ng/tube×2 |
| FEK0663-A | Mouse CD40 Antibody A | 3ml |
| FEK0703-B | Mouse CD40 Antibody B | 3ml |
| AR1106-1 | Sample Diluent | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 28 | 198 | 433 | 32 | 196 | 412 |
| Standard deviation | 1.54 | 8.91 | 26.41 | 1.22 | 13.52 | 23.07 |
| CV (%) | 5.5% | 4.5% | 6.1% | 3.8% | 6.9% | 5.6% |
典型数据示例
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.033 | 0.159 | 0.249 | 0.448 | 0.785 | 1.405 | 1.981 | 2.319 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)