产品名称
Mouse TNFRSF5/CD40 ELISA Kit
指标别名
B cell surface antigen CD40; Bp50; CD40; CD40L receptor; CDW40; p50; TNFRSF5
免疫原
Expression system for standard: NS0; Immunogen sequence: V24-R193
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tumor necrosis factor receptor superfamily member 5
文献引用格式
Mouse TNFRSF5/CD40 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0703)
背景资料
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0703-CAP | 96-well plate precoated with anti- mouse CD40 antibody | 1 |
| EK0703-ST | Lyophilized recombinant mouse CD40 standard | 10ng/tube×2 |
| EK0703-DA | Biotinylated anti- mouse CD40 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 30 | 208 | 421 | 27 | 195 | 393 |
| Standard deviation | 1.86 | 10.81 | 18.1 | 1.86 | 12.09 | 22.79 |
| CV(%) | 6.2% | 5.2% | 4.3% | 6.9% | 6.2% | 5.8% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.036 | 0.071 | 0.104 | 0.172 | 0.294 | 0.52 | 0.977 | 1.838 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)