产品名称
Human TNFRSF5/CD40 Quick ELISA Kit
指标别名
B cell surface antigen CD40; Bp50; CD40; CD40L receptor; CDW40; p50; TNFRSF5
免疫原
Expression system for standard: NS0; Immunogen sequence: E21-R193
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
CD40 molecule, TNF receptor superfamily member 5
文献引用格式
Human TNFRSF5/CD40 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0702)
背景资料
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
试剂盒内容
| Catalog number | Description | Quantity |
| FEK0663-CAP | 96-well plate precoated with anti-Human TNFRSF5 antibody | 1 |
| FEK0663-ST | lyophilized recombinant Human TNFRSF5 standard | 10ng/tube×2 |
| FEK0663-A | Human TNFRSF5 Antibody A | 3ml |
| FEK0702-B | Human TNFRSF5 Antibody B | 3ml |
| AR1106-1 | Sample Diluent | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 165 | 415 | 34 | 181 | 409 |
| Standard deviation | 1.49 | 7.92 | 16.19 | 2.01 | 11.22 | 35.99 |
| CV (%) | 4.5% | 4.8% | 3.9% | 5.9% | 6.2% | 8.8% |
典型数据示例
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.031 | 0.114 | 0.180 | 0.315 | 0.559 | 0.991 | 1.694 | 2.208 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk