Human Lipocalin-2/NGAL/LCN2 ELISA Kit

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EK0853

96T ￥2480
货期：现货

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Human Lipocalin-2/NGAL ELISA Kit standard curve
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产品简介>

产品名称

Human Lipocalin-2/NGAL/LCN2 ELISA Kit

规格/价格

96T/2480

指标别名

lipocalin 2; 24p3; MSFI; NGAL; p25; LCN2

免疫原

NSO, Q21-G198

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156 pg/ml - 10000 pg/ml

基因名称

LCN2

基因名全称

lipocalin 2

Uniprot ID

P80188

RRID

AB_2800331

文献引用格式

Human Lipocalin-2/NGAL/LCN2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0853)

背景资料

Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.The standard product used in this kit is recombinant human NGAL, consisting of 178 amino acids with the molecular mass of 22KDa.

显色时间

15-20分钟

试剂盒内容

Catalog number	Description	Quantity
EK0853-CAP	96-well plate precoated with anti- human NGAL antibody	1
EK0853-ST	Lyophilized recombinant human NGALstandard	10ng/tube×2
EK0853-DA	Biotinylated anti- human NGAL antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	256	1510	5422	254	1537	5138
Standard deviation	19.2	81.54	281.94	19.05	84.53	323.69
CV(%)	7.5	5.4	5.2	7.5	5.5	6.3

典型数据示例

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.040	0.317	0.479	0.780	1.049	1.588	1.877	2.090

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human Lipocalin-2/NGAL/LCN2 ELISA Kit

EK0853

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

RRID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

实验时长

其他介绍>

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Human Lipocalin-2/NGAL/LCN2 ELISA Kit

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