产品名称
Human Lipocalin-2/NGAL/LCN2 EZ-Set™ ELISA Kit (DIY Antibody Pairs)
指标别名
lipocalin 2; 24p3; MSFI; NGAL; p25; LCN2
免疫原
Expression system for standard: NS0; Immunogen sequence: Q21-G198
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10,000 pg/ml
文献引用格式
Human Lipocalin-2/NGAL/LCN2 EZ-Set™ ELISA Kit (DIY Antibody Pairs) (Boster Biological Technology, Wuhan, China. Catalog#EZ0853)
背景资料
Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase. The LCN2 gene is located at 9q34 and contains 7 exons. The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions. The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long. LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung.The standard product used in this kit is recombinant human NGAL, consisting of 178 amino acids with the molecular mass of 22KDa.
试剂盒内容
| Catalog number |
Description |
Quantity |
Storage of opened/reconstituted material |
| EZ0853-CA |
Rabbit anti- human LCN2 polyclonal antibody (Capture Antibody) |
500 µl, 0.4 mg/mL
(recommended dilution 1:100) |
Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
|
| EZ0853-DA |
Biotinylated goat anti- human LCN2 polyclonal antibody (Detection Antibody) |
500 µl, 4.7 µg/mL
(recommended dilution 1:100) |
| AR1103 |
Avidin-Biotin-Peroxidase Complex (ABC) |
500 µl (recommended dilution 1:100) |
| EZ853-ST |
Lyophilized recombinant human LCN2 standard |
10 ng/tube×3
|
Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the
expiration date of the kit. |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.04 | 0.317 | 0.479 | 0.78 | 1.049 | 1.588 | 1.877 | 2.09 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
需要自备的试剂
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution:
Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12.Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
*样品准备的相关试剂也不含在试剂盒中。
蛋白名全称
Neutrophil gelatinase-associated lipocalin
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin), Saliva and Urine
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