Rat Lipocalin-2/NGAL/LCN2 ELISA Kit

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EK0855

96T ￥2480
货期：现货

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Rat Lipocalin-2/NGAL ELISA Kit standard curve
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产品简介>

产品名称

Rat Lipocalin-2/NGAL/LCN2 ELISA Kit

规格/价格

96T/2480

指标别名

lipocalin 2; 24p3; MSFI; NGAL; p25; LCN2

免疫原

Expression system for standard: NS0; Immunogen sequence: Q21-N198

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

78pg/ml-5000pg/ml

基因名称

LCN2

基因名全称

lipocalin 2

Uniprot ID

P30152

文献引用格式

Rat Lipocalin-2/NGAL/LCN2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0855)

背景资料

Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kDa.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK0855-CAP	96-well plate precoated with anti- rat NGAL antibody	1
EK0855-ST	Lyophilized recombinant rat NGAL standard	10ng/tube×2
EK0855-DA	Biotinylated anti- rat NGAL antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	121	750	3188	126	727	3167
Standard deviation	6.05	50.25	168.96	7.3	56.7	177.35
CV(%)	5%	6.7%	5.3%	5.8%	7.8%	5.6%

典型数据示例

Concentration(pg/ml)	0	78	156	313	625	1250	2500	5000
O.D.	0.096	0.134	0.168	0.232	0.359	0.604	1.129	1.918

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Rat Lipocalin-2/NGAL/LCN2 ELISA Kit

EK0855

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

检验物种

检测方法

样本类型

其他介绍>

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Rat Lipocalin-2/NGAL/LCN2 ELISA Kit

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