产品名称
Mouse Lipocalin-2/NGAL/LCN2 Quick ELISA Kit
指标别名
lipocalin 2; 24p3; MSFI; NGAL; p25; Lcn2
免疫原
Expression system for standard: NS0; Immunogen sequence: Q21-N200
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Lipocalin-2/NGAL/LCN2 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0854)
背景资料
Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22KDa.
试剂盒内容
Catalog number | Description | Quantity |
FEK0854-CAP | Anti-mouse NGAL Pre-coated 96-well Strip Microplate | 1 |
FEK0854-ST | mouse NGAL standard | 10ng/tube×2 |
FEK0854-H | HRP Linked Anti-mouse NGAL Antibody | 6ml |
AR1106-1 | Sample diluent buffer | 15ml |
AR1106-7 | TBS-T Wash Buffer (25x) | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | Stop solution | 10ml |
PLA-SEA | Adhesive cover | 2 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 141 | 785 | 2588 | 130 | 817 | 2606 |
Standard deviation | 6.2 | 38.46 | 113.87 | 7.54 | 54.73 | 161.57 |
CV(%) | 4.4% | 4.9% | 4.4% | 5.8% | 6.7% | 6.2% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.014 | 0.131 | 0.237 | 0.425 | 0.807 | 1.353 | 1.961 | 2.347 |
保存条件与有效期
Store at 4℃ for 6 months.
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine