Anti-HNF4A Antibody (Clone#6C8E9)

Mouse monoclonal antibody

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筛选器： All WB IHC

M00389-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HNF4A using anti-HNF4A antibody (M00389-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HEPG2 whole cell lysates,
Lane 2: rat liver ttissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-HNF4A antigen affinity purified monoclonal antibody (M00389-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HNF4A at approximately 53 kDa. The expected band size for HNF4A is at 53 kDa.
all(3)
IHC analysis of HNF4A using anti-HNF4A antibody (M00389-2).
HNF4A was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with mouse anti-HNF4A Antibody (M00389-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of HNF4A using anti-HNF4A antibody (M00389-2).
HNF4A was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with mouse anti-HNF4A Antibody (M00389-2) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-HNF4A Antibody (Clone#6C8E9)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

hepatocyte nuclear factor 4 alpha; FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14; HNF4A

产品类型

Monoclonal

检验物种

human, rat

应用范围

WB, IHC

基因名称

HNF4A

克隆号

6C8E9

宿主

Mouse

抗体亚型

IgG2a

免疫原

E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequence identity with mouse and rat HNF-4-alpha, respectively.

计算分子量

53 kDa

实际分子量

53 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.

Uniprot ID

P41235

文献引用格式

HNF4A Antibody (Clone#6C8E9) (Boster Biological Technology, Wuhan, China. Catalog#M00389-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

hepatocyte nuclear factor 4, alpha

蛋白名全称

Hepatocyte nuclear factor 4-alpha

实验方案>

实验步骤

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