| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |

Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human U937 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody (PB9663) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lysozyme/LYZ at approximately 15-17 kDa. The expected band size for Lysozyme/LYZ is at 17 kDa.

IHC analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663) .
Lysozyme/LYZ was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IF analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663).
Lysozyme/LYZ was detected in a paraffin-embedded section of human ileum tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663).
Lysozyme/LYZ was detected in a paraffin-embedded section of human colon organoid tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663).
Lysozyme/LYZ was detected in a paraffin-embedded section of mouse ileum tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663).
Lysozyme/LYZ was detected in a paraffin-embedded section of mouse ileum organoid tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human U937 whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody (PB9663) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lysozyme/LYZ at approximately 15-17 kDa. The expected band size for Lysozyme/LYZ is at 17 kDa.

IHC analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663) .
Lysozyme/LYZ was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IF analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663).
Lysozyme/LYZ was detected in a paraffin-embedded section of human ileum tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663).
Lysozyme/LYZ was detected in a paraffin-embedded section of human colon organoid tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663).
Lysozyme/LYZ was detected in a paraffin-embedded section of mouse ileum tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (PB9663).
Lysozyme/LYZ was detected in a paraffin-embedded section of mouse ileum organoid tissue. The tissue section was incubated with rabbit anti-Lysozyme/LYZ Antibody (PB9663) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).





