Anti-14-3-3 Epsilon/YWHAE Antibody (Clone#3G11G2)

筛选器： All WB IHC FCM ICC/IF

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  Western blot analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: SH-SY5Y whole cell lysates,
  Lane 3: SW620 whole cell lysates,
  Lane 4: Raji whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: PC-12 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-14-3-3 Epsilon/YWHAE antigen affinity purified monoclonal antibody (M01687-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 Epsilon/YWHAE at approximately 29 kDa. The expected band size for 14-3-3 Epsilon/YWHAE is at 29 kDa.

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  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human Colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human endometrial cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of ANA-1 cells using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  Overlay histogram showing ANA-1 cells stained with M01687-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of NRK cells using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  Overlay histogram showing NRK cells stained with M01687-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody (M01687-2).
  14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with mouse anti-14-3-3 Epsilon/YWHAE Antibody (M01687-2) at a dilution of 1:100. Dylight594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1141) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Anti-a-SMA/ACTA2 Antibody (Clone#1A4)

筛选器： All WB IHC IF

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  Western blot analysis of anti-α-SMA antibody (BM0002). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: rat stomach tissue lysates,
  Lane 3: mouse stomach tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-α-SMA antigen affinity purified polyclonal antibody (BM0002) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for α-SMA at approximately 42 kDa. The expected band size for α-SMA is at 42 kDa.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human endometrial carcinoma tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of human placenta tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of mouse pancreas tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of rat heart muscle tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of rat lung tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with AEC (Catalog # AR1020) as the chromogen.

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  IHC analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of rat liver tissue. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of α-SMA using anti-α-SMA antibody (BM0002).
  α-SMA was detected in a paraffin-embedded section of rat lung tissue. Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  IF analysis of α-SMA using anti-α-SMA antibody (BM0002) and anti Anti-PECAM-1/CD31 antibody (PB9094).
  α-SMA was detected in a paraffin-embedded section of human placenta tissue. Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog#BA1126) and Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Anti-A26C2/POTEG Antibody (Clone#OTI2B5)

筛选器： All WB

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POTEG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POTEG (1:2000).

Anti-A2BP1/RBFOX1 Antibody (Clone#OTI1F2)

筛选器： All WB

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBFOX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBFOX1.

Anti-AANAT Antibody (Clone#OTI6E1)

筛选器： All WB

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AANAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AANAT (1:2000).

Anti-ABAT Antibody (Clone#OTI3D2)

筛选器： All WB IHC

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABAT.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M07133-3)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M07133-3)

Anti-ABCF2 Antibody (Clone#OTI8F4)

筛选器： All WB IHC

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and ABCF2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 monoclonal antibody M10873, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody ([MA01263]) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ABCF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M10873) (1:500)

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-ABCF2 monoclonal antibody (1:500).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCF2 (Cat# M10873)(1:2000).

Anti-ACAA2 Antibody (Clone#OTI1D3)

筛选器： All WB IHC ICC/IF

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAA2.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M08341-2)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M08341-2)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M08341-2)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M08341-2)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACAA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M08341-2)

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  Immunofluorescent staining of HeLa cells using anti-ACAA2 mouse monoclonal antibody.

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  Anti-ACAA2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAA2 .

Anti-ACADM Antibody (Clone#OTI2G7)

筛选器： All WB IHC

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACADM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACADM (Cat# M02383-1)(1:2000).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M02383-1) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M02383-1) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M02383-1) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M02383-1) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M02383-1) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M02383-1) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M02383-1) (1:500)

Anti-ACAT2 Antibody (Clone#OTI3A11)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.

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  Immunofluorescent staining of HT29 cells using anti-ACAT2 mouse monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

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  HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody, and then analyzed by flow cytometry.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03245-3, Dilution 1:50)

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  Anti-ACAT2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 .

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.

Anti-ACBD3 Antibody (Clone#OTI1G2)

筛选器： All WB IHC FCM

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACBD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACBD3.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ACBD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

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  HEK293T cells transfected with either ACBD3 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACBD3 antibody (M05645)

Anti-ACE2 Antibody (Clone#OTI4C5)

筛选器： All WB IHC

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACE2 (MA00756). (1:500)

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  IHC analysis of ACE2 using anti-ACE2 antibody (MA00756).
  ACE2 was detected in a paraffin-embedded section of human renal cancer tissue. The tissue section was incubated with mouse anti-ACE2 Antibody (MA00756) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.

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Anti-Acetyl CoA synthetase/ACSS2 Antibody (Clone#OTI3A8)

筛选器： All IHC WB

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ACSS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02809-2)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSS2.

Anti-Acetyl-Alpha Tubulin/TUBA1B (Lys40) Antibody (Clone#OTI3H2)

筛选器： All WB

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA1B.

Anti-ACLY Antibody (Clone#5I2)

筛选器： All WB IHC FCM ICC/IF

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  Western blot analysis of ACLY using anti-ACLY antibody (M02372-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Human placenta tissue lysates,
  Lane 2: U-87MG whole cell lysates,
  Lane 3: HEK293 whole cell lysates,
  Lane 4: Caco-2 whole cell lysates,
  Lane 5: HL-60 whole cell lysates,
  Lane 6: Raji whole cell lysates,
  Lane 7: THP-1 whole cell lysates,
  Lane 8: PANC-1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ACLY antigen affinity purified monoclonal antibody (M02372-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACLY at approximately 121 kDa. The expected band size for ACLY is at 121 kDa.

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  Western blot analysis of ACLY using anti-ACLY antibody (M02372-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Rat lung tissue lysates,
  Lane 2: Rat testicular tissue lysates,
  Lane 3: Rat kidney tissue lysates,
  Lane 4: Rat brain tissue lysates,
  Lane 5: Mouse lung tissue lysates,
  Lane 6: Mouse testicular tissue lysates,
  Lane 7: Mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ACLY antigen affinity purified monoclonal antibody (M02372-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACLY at approximately 121 kDa. The expected band size for ACLY is at 121 kDa.

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  IHC analysis of ACLY using anti-ACLY antibody (M02372-1).
  ACLY was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACLY Antibody (M02372-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of ACLY using anti-ACLY antibody (M02372-1).
  ACLY was detected in a paraffin-embedded section of human testis cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACLY Antibody (M02372-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of ACLY using anti-ACLY antibody (M02372-1).
  ACLY was detected in a paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACLY Antibody (M02372-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of ACLY using anti-ACLY antibody (M02372-1).
  ACLY was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACLY Antibody (M02372-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  Flow cytometry analysis of A549 cell(1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).

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  IF analysis of ACLY using anti-ACLY antibody (M02372-1).
  ACLY was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with mouse anti-ACLY Antibody (M02372-1) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Anti-ACLY Antibody (Clone#OTI3G8)

筛选器： All WB IHC ICC/IF

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  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-ACLY monoclonal antibody (1:200).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACLY (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACLY(Cat# M02372-2).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ACLY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02372-2, Dilution 1:50)

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  Anti-ACLY mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACLY .

Anti-Aconitase 2/ACO2 Antibody (Clone#4C12D1)

筛选器： All WB IHC

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  Western blot analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: U-87MG whole cell lysates,
  Lane 3: Jurkat whole cell lysates,
  Lane 4: SH-SY5Y whole cell lysates,
  Lane 5: rat skeletal muscle tissue lysates,
  Lane 6: rat brain tissue lysates,
  Lane 7: mouse skeletal muscle tissue lysates,
  Lane 8: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Aconitase 2/ACO2 antigen affinity purified monoclonal antibody (M03096-3) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Aconitase 2/ACO2 at approximately 85 kDa. The expected band size for Aconitase 2/ACO2 is at 85 kDa.

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  IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
  Aconitase 2/ACO2 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
  Aconitase 2/ACO2 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
  Aconitase 2/ACO2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
  Aconitase 2/ACO2 was detected in a paraffin-embedded section of human Lung adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
  Aconitase 2/ACO2 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
  Aconitase 2/ACO2 was detected in a paraffin-embedded section of rat heart tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Aconitase 2/ACO2 using anti-Aconitase 2/ACO2 antibody (M03096-3).
  Aconitase 2/ACO2 was detected in a paraffin-embedded section of mouse heart tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Aconitase 2/ACO2 Antibody (M03096-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

Anti-Aconitase 2/ACO2 Antibody (Clone#OTI3G8)

筛选器： All IHC ICC/IF WB

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-ACO2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03096, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ACO2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03096, Dilution 1:50)

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  Anti-ACO2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACO2 .

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ACO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M03096, Dilution 1:50)

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ACO2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunofluorescent staining of HepG2 cells using anti-ACO2 mouse monoclonal antibody.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACO2(Cat# M03096).

Anti-ACOT12 Antibody (Clone#OTI1A11)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA11589)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA11589)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA11589)

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  HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACOT12 antibody, and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-ACOT12 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA11589)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA11589)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACOT12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA11589)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACOT12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACOT12(Cat# MA11589).

Anti-ACSL4/FACL4 Antibody (Clone#4I7)

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HepG2 whole cell lysates,
  Lane 3: PC-3 whole cell lysates,
  Lane 4: Hela whole cell lysates,
  Lane 5: Caco-2 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ACSL4/FACL4 antigen affinity purified monoclonal antibody (M04372) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACSL4/FACL4 at approximately 79 kDa. The expected band size for ACSL4/FACL4 is at 79 kDa.

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  IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
  ACSL4/FACL4 was detected in a paraffin-embedded section of human Bladder epithelial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
  ACSL4/FACL4 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
  ACSL4/FACL4 was detected in a paraffin-embedded section of human Metaplasia of squamous cells of the renal pelvis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
  ACSL4/FACL4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
  ACSL4/FACL4 was detected in a paraffin-embedded section of human Rectal moderately differentiatedadenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of ACSL4/FACL4 using anti-ACSL4/FACL4 antibody (M04372).
  ACSL4/FACL4 was detected in an immunocytochemical section of SiHa cells. The section was incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of HepG2 cells using anti-ACSL4/FACL4 antibody (M04372).
  Overlay histogram showing HepG2 cells stained with M04372 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ACSL4/FACL4 Antibody (M04372) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.