Western blot analysis of SMAD1 using anti-SMAD1 antibody (PB0444). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Cardiac Muscle tissue lysates,
Lane 2: Mouse Cardiac Muscle tissue lysates,
Lane 3: Rat Skeletal Muscle tissue lysates,
Lane 4: Mouse Skeletal Muscle tissue lysates,
Lane 5: 293T whole cell lysates,
Lane 6: MCF-7 whole cell lysates,
Lane 7: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (PB0444) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD1 at approximately 52 kDa. The expected band size for SMAD1 is at 52 kDa.

Western blot analysis of SMAD3 using anti-SMAD3 antibody (PB0445). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: U87 whole cell lysates,
Lane 2: Human Placenta tissue lysates,
Lane 3: PANC whole cell lysates,
Lane 4: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD3 antigen affinity purified polyclonal antibody (PB0445) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD3 at approximately 58 kDa. The expected band size for SMAD3 is at 48 kDa.

Western blot analysis of SMAD4 using anti-SMAD4 antibody (PB0446). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: Mouse Brain tissue lysates,
Lane 3: Rat Skeletal Muscle tissue lysates,
Lane 4: Mouse Skeletal Muscle tissue lysates,
Lane 5: U87 whole cell lysates,
Lane 6: Human Placenta tissue lysates,
Lane 7: HT1080 whole cell lysates,
Lane 8: HELA whole cell lysates,
Lane 9: NEURO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD4 antigen affinity purified polyclonal antibody (PB0446) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD4 at approximately 60 kDa. The expected band size for SMAD4 is at 60 kDa.

Western blot analysis of SMN1/2 using anti-SMN1/2 antibody (PB0447). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: Mouse Brain tissue lysates,
Lane 3: Rat Liver tissue lysates,
Lane 4: Mouse Liver tissue lysates,
Lane 5: 293T whole cell lysates,
Lane 6: SMMC whole cell lysates,
Lane 7: HEPG2 whole cell lysates,
Lane 8: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMN1/2 antigen affinity purified polyclonal antibody (PB0447) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMN1/2 at approximately 39 kDa. The expected band size for SMN1/2 is at 32 kDa.

IHC analysis of SMN1/2 using anti-SMN1/2 antibody (PB0447).
SMN1/2 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SMN1/2 Antibody (PB0447) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of SMN1/2 using anti-SMN1/2 antibody (PB0447).
SMN1/2 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SMN1/2 Antibody (PB0447) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of SMN1/2 using anti-SMN1/2 antibody (PB0447).
SMN1/2 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SMN1/2 Antibody (PB0447) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IF analysis of SMN1/2 using anti-SMN1/2 antibody (PB0447).
SMN1/2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SMN1/2 Antibody (PB0447) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Flow Cytometry analysis of A431 cells using anti-SMN1/2 antibody (PB0447).
Overlay histogram showing A431 cells stained with PB0447 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMN1/2 Antibody (PB0447) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of SNAI1 using anti-SNAI1 antibody (PB0449). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human SMMC-7721 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNAI1 antigen affinity purified polyclonal antibody (PB0449) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SNAI1 at approximately 29 kDa. The expected band size for SNAI1 is at 29 kDa.

Western blot analysis of SNAI3 using anti-SNAI3 antibody (PB0448). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNAI3 antigen affinity purified polyclonal antibody (PB0448) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SNAI3 at approximately 72 kDa. The expected band size for SNAI3 is at 32 kDa.

Western blot analysis of Serotonin transporter/SLC6A4 using anti-Serotonin transporter/SLC6A4 antibody (PB0442). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Serotonin transporter/SLC6A4 antigen affinity purified polyclonal antibody (PB0442) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Serotonin transporter/SLC6A4 at approximately 70-90 kDa. The expected band size for Serotonin transporter/SLC6A4 is at 70 kDa.

Western blot analysis of Serotonin transporter/SLC6A4 using anti-Serotonin transporter/SLC6A4 antibody (PB0442). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Serotonin transporter/SLC6A4 antigen affinity purified polyclonal antibody (PB0442) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Serotonin transporter/SLC6A4 at approximately 70-90 kDa. The expected band size for Serotonin transporter/SLC6A4 is at 70 kDa.

Western blot analysis of Serotonin transporter/SLC6A4 using anti-Serotonin transporter/SLC6A4 antibody (PB0442). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Serotonin transporter/SLC6A4 antigen affinity purified polyclonal antibody (PB0442) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Serotonin transporter/SLC6A4 at approximately 70-90 kDa. The expected band size for Serotonin transporter/SLC6A4 is at 70 kDa.

IHC analysis of Serotonin transporter/SLC6A4 using anti-Serotonin transporter/SLC6A4 antibody (PB0442).
Serotonin transporter/SLC6A4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Serotonin transporter/SLC6A4 Antibody (PB0442) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Serotonin transporter/SLC6A4 using anti-Serotonin transporter/SLC6A4 antibody (PB0442).
Serotonin transporter/SLC6A4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Serotonin transporter/SLC6A4 Antibody (PB0442) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IF analysis of Serotonin transporter/SLC6A4 using anti-Serotonin transporter/SLC6A4 antibody (PB0442).
Serotonin transporter/SLC6A4 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-Serotonin transporter/SLC6A4 Antibody (PB0442) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

IF analysis of Serotonin transporter/SLC6A4 using anti-Serotonin transporter/SLC6A4 antibody (PB0442).
Serotonin transporter/SLC6A4 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-Serotonin transporter/SLC6A4 Antibody (PB0442) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).