Anti-CA15-3/MUC1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF ELISA

A00187-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CA15-3/MUC1 using anti-CA15-3/MUC1 antibody (A00187-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T-47D whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CA15-3/MUC1 antigA03957-Aen affinity purified polyclonal antibody (A00187-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CA15-3/MUC1 at approximately 25-30 kDa. The expected band size for CA15-3/MUC1 is at 122 kDa.
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IHC analysis of CA15-3/MUC1 using anti-CA15-3/MUC1 antibody (A00187-2).
CA15-3/MUC1 was detected in a paraffin-embedded section of human invasive adenocarcinoma of the lung tissue. The tissue section was incubated with rabbit anti-CA15-3/MUC1 Antibody (A00187-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of CA15-3/MUC1 using anti-CA15-3/MUC1 antibody (A00187-2).
CA15-3/MUC1 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. The tissue section was incubated with rabbit anti-CA15-3/MUC1 Antibody (A00187-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of CA15-3/MUC1 using anti-CA15-3/MUC1 antibody (A00187-2).
CA15-3/MUC1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-CA15-3/MUC1 Antibody (A00187-2) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

Anti-CA15-3/MUC1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CA15 3; CA153; CA15-3; Carcinoma associated mucin; CD227; EMA; Episialin; H23AG; KL-6; MAM6; MUC 1; MUC1; MUC1 beta; MUC1 CT; MUC1 NT; MUC1/CA15-3 C-terminal; Mucin 1; Peanut reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor associated mucin

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, IF, ELISA

基因名称

MUC1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human MUC1 recombinant protein (Position: T1063-R1131). Human MUC1 shares 59.1% and 58.5% amino acid (aa) sequence identity with mouse and rat MUC1, respectively.

计算分子量

122 kDa

实际分子量

25-30 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. It is mapped to 1q22. Mucin 1 is a transmembrane mucin normally expressed on the apical borders of secretory epithelial cells. Overexpression of Mucin 1 is often associated with colon, breast, ovarian, lung and pancreatic cancers. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Mucin 1 stimulated ESR1-mediated transcription and contributed to estradiol-mediated growth and survival of breast cancer cells. This gene also can suppress pulmonary innate immunity, and its antiinflammatory activity may play an important modulatory role during microbial infection.

Uniprot ID

P15941

RRID

AB_3081005

文献引用格式

CA15-3/MUC1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00187-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

MUC1 mucin 1, cell surface associated

蛋白名全称

Mucin-1

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