Anti-HSP27/HSPB1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00676

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HSP27/HSPB1 using anti-HSP27/HSPB1 antibody (A00676). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP27/HSPB1 antigen affinity purified polyclonal antibody (A00676) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSP27/HSPB1 at approximately 27 kDa. The expected band size for HSP27/HSPB1 is at 23 kDa.
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IHC analysis of HSP27/HSPB1 using anti-HSP27/HSPB1 antibody (A00676).
HSP27/HSPB1 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSP27/HSPB1 Antibody (A00676) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of HSP27/HSPB1 using anti-HSP27/HSPB1 antibody (A00676).
HSP27/HSPB1 was detected in a paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSP27/HSPB1 Antibody (A00676) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of HSP27/HSPB1 using anti-HSP27/HSPB1 antibody (A00676).
HSP27/HSPB1 was detected in a paraffin-embedded section of mouse small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSP27/HSPB1 Antibody (A00676) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
all(5)

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产品简介>

产品名称

Anti-HSP27/HSPB1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Heat shock protein beta 1; HSP 27; Hsp25; HSP27; HSP28; HSPB1

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ELISA

基因名称

HSPB1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse Hsp27 recombinant protein (Position: M1-K209).

计算分子量

23 kDa

实际分子量

27 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

HSPB1 (Heat shock 27kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. It is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Uniprot ID

P14602

文献引用格式

HSP27/HSPB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00676)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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