产品名称
Anti-HSP27/HSPB1 Antibody (Clone#IAP-9)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
Heat shock protein beta 1; HSP 27; Hsp25; HSP27; HSP28; HSPB1
检验物种
human, mouse, rat, rabbit
免疫原
Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.
成分
200 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
保存条件
12 months from date of receipt，-20℃ as supplied.
背景资料
The heat-shock proteins(HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small(27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
文献引用格式
HSP27/HSPB1 Antibody (Clone#IAP-9) (Boster Biological Technology, Wuhan, China. Catalog#BM1586)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
heat shock 27kDa protein 1
蛋白名全称
Heat shock protein beta-1
推荐稀释比
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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