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Anti-C-MYC/MYC Antibody (Clone#9E10)

Mouse monoclonal antibody

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筛选器: All WB IHC ICC/IF

BM0238

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti- XCR1 antibody (BM0238). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: Hela whole cell lysates.
    Use mouse anti- XCR1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for XCR1 at approximately 49KD. The expected band size for XCR1 is at 49KD.

    all(5)
  • IHC analysis using anti- XCR1 antibody (BM0238). detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(5)
  • IHC analysis using anti- XCR1 antibody (BM0238). detected in paraffin-embedded section of human spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(5)
  • IHC analysis using anti- XCR1 antibody (BM0238). detected in paraffin-embedded section of human thyroid cance tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

    all(5)

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产品名称
Anti-C-MYC/MYC Antibody (Clone#9E10)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
bHLHe39; c Myc; c-Myc; MRTL; MYC; Myc proto oncogene protein; Proto oncogene c Myc; Transcription factor p64
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF
基因名称
MYC
克隆号
9E10
宿主
Mouse
抗体亚型
IgG1
免疫原
Polypeptide
计算分子量
49 kDa
实际分子量
49 kDa
成分
200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
200ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
Uniprot ID
P01106  
文献引用格式
C-MYC/MYC Antibody (Clone#9E10) (Boster Biological Technology, Wuhan, China. Catalog#BM0238)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
基因名全称
v-myc myelocytomatosis viral oncogene homolog(avian)
蛋白名全称
Myc proto-oncogene protein
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-C-MYC/MYC Antibody (Clone#9E10)

    筛选器: All WB IHC ICC/IF

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