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Anti-C-MYC/MYC Antibody (Clone#OTI1A6)

Mouse monoclonal antibody

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筛选器: All WB IHC FCM ICC/IF

MA00026

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MYC monoclonal antibody (1:200).

    all(19)
  • Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-MYC monoclonal antibody at 1:200 dilution.

    all(19)
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYC(Cat# M00026-4).

    all(19)
  • Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MYC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00026-4)

    all(19)
  • HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYC antibody, and then analyzed by flow cytometry.

    all(19)
  • Anti-MYC mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYC .

    all(19)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-C-MYC/MYC Antibody (Clone#OTI1A6)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
bHLHe39; c Myc; c-Myc; MRTL; MYC; Myc proto oncogene protein; Proto oncogene c Myc; Transcription factor p64
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, FCM, ICC/IF
基因名称
MYC
克隆号
OTI1A6
宿主
Mouse
抗体亚型
IgG2b
免疫原
Full length human recombinant protein of human Myc (NP_002458) produced in E.coli.
计算分子量
49 kDa
实际分子量
50.4 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
MYC proto-oncogene, bHLH transcription factor is a protein that in humans is encoded by the MYC gene which is a member of the myc family of transcription factors. The protein contains basic helix-loop-helix (bHLH) structural motif. This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Uniprot ID
P01106  
文献引用格式
C-MYC/MYC Antibody (Clone#OTI1A6) (Boster Biological Technology, Wuhan, China. Catalog#MA00026)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
MYC proto-oncogene, bHLH transcription factor
蛋白名全称
Myc proto-oncogene protein
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:50
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100
Flow cytometry (FCM):1:100

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    Anti-C-MYC/MYC Antibody (Clone#OTI1A6)

    筛选器: All WB IHC FCM ICC/IF

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