Anti-C-MYC/MYC Antibody (Clone#AHI-13)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4042

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-C-MYC/MYC antibody (BM4042). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C-MYC/MYC antigen affinity purified monoclonal antibody (BM4042) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for C-MYC/MYC at approximately 57 kDa. The expected band size for C-MYC/MYC is at 49 kDa.
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Immunohistochemical analysis of paraffin-embedded human lung cancer, using c-Myc Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of HeLa cells, using c-Myc Antibody.
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产品名称

Anti-C-MYC/MYC Antibody (Clone#AHI-13)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

bHLHe39; c Myc; c-Myc; MRTL; MYC; Myc proto oncogene protein; Proto oncogene c Myc; Transcription factor p64

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

MYC

克隆号

AHI-13

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human c-Myc Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Myc a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors.. Seems to activate the transcription of growth-related genes.

计算分子量

49 kDa

实际分子量

57 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Uniprot ID

P01106

文献引用格式

C-MYC/MYC Antibody (Clone#AHI-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4042)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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