Anti-C-MYC/MYC (Phospho-T58) Antibody (Clone#FOC-13)

Rabbit Monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4469

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-P-Myc antibody (BM4469). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-Myc antigen affinity purified monoclonal antibody (BM4469) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-Myc at approximately 57 kDa. The expected band size for P-Myc is at 49 kDa.
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IHC analysis of P-Myc using anti-P-Myc antibody (BM4469).
P-Myc was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-P-Myc Antibody (BM4469) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of P-Myc using anti-P-Myc antibody (BM4469).
P-Myc was detected in a paraffin-embedded section of human skin cancer tissue. The tissue section was incubated with rabbit anti-P-Myc Antibody (BM4469) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-C-MYC/MYC (Phospho-T58) Antibody (Clone#FOC-13)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

bHLHe39; c Myc; c-Myc; MRTL; MYC; Myc proto oncogene protein; Proto oncogene c Myc; Transcription factor p64

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

MYC

克隆号

FOC-13

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-c-Myc (T58)

计算分子量

49 kDa

实际分子量

57 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Uniprot ID

P01106

文献引用格式

C-MYC/MYC (Phospho-T58) Antibody (Clone#FOC-13) (Boster Biological Technology, Wuhan, China. Catalog#BM4469)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Myc proto-oncogene protein

实验方案>

实验步骤

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