Anti-C-MYC/MYC Antibody

Rabbit polyclonal antibody

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筛选器： All WB ICC/IF

PB9092

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-C-MYC antibody (PB9092). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C-MYC antigen affinity purified polyclonal antibody (PB9092) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for C-MYC at approximately 60-65 kDa. The expected band size for C-MYC is at 49 kDa.
all(2)
IF analysis of C-MYC using anti-C-MYC antibody (PB9092) and anti-Beta Tubulin antibody (M01857-3).
C-MYC was detected in an immunocytochemical section of Hela cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
all(2)

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产品名称

Anti-C-MYC/MYC Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

bHLHe39; c Myc; c-Myc; MRTL; MYC; Myc proto oncogene protein; Proto oncogene c Myc; Transcription factor p64

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, ICC/IF

基因名称

MYC

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human c-Myc recombinant protein (Position: E257-A439). Human c-Myc shares 91% amino acid (aa) sequences identity with both mouse and rat c-Myc.

计算分子量

49 kDa

实际分子量

57-65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Uniprot ID

P01106

RRID

AB_3081966

文献引用格式

C-MYC/MYC Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9092)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Myc proto-oncogene protein

蛋白名全称

Myc proto-oncogene protein

实验方案>

实验步骤

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