Anti-IRF3 Antibody
送常用试剂

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF IP FCM ELISA

A00165-7

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of anti-IRF3 antibody (A00165-7). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IRF3 antigen affinity purified polyclonal antibody (A00165-7) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IRF3 at approximately 50-55 kDa. The expected band size for IRF3 is at 47 kDa.
all(7)
IHC analysis of IRF3 using anti-IRF3 antibody (A00165-7) .
IRF3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-IRF3 Antibody (A00165-7) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of IRF3 using anti-IRF3 antibody (A00165-7) .
IRF3 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-IRF3 Antibody (A00165-7) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(7)
ICC/IF analysis of IRF3 using anti-IRF3 antibody (A00165-7).
IRF3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-IRF3 Antibody (A00165-7) at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(7)
all(7)
all(7)
all(7)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IRF3 Antibody

规格/价格

50μl|1280元
100μl|2180元
150μl|2800元

指标别称

interferon regulatory factor 3; IRF 3; IRF3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM, ELISA

基因名称

IRF3

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human IRF3 recombinant protein (Position: T3-L450).

计算分子量

47 kDa

实际分子量

50-55 kDa

成分

500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

IRF3(interferon regulatory factor 3)?is a member of the interferon regulatory transcription factor(IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with?CREBBP. IRF3 plays an important role in the?innate immune system's response to?viral infection. Aggregated?MAVS?have been found to activate IRF3 dimerization. Although?IRF3?increased transcriptional activity from an ISRE-containing promoter, expression of?IRF3?as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase(CAT) reporter gene containing repeats of the Gal4-binding sites.?Translocation of?IRF3?was accompanied by an increase in serine and threonine phosphorylation.?The transcriptional activators CREBBP and EP300 coimmunoprecipitated with?IRF3?only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Uniprot ID

Q14653

文献引用格式

IRF3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00165-7)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interferon regulatory factor 3

蛋白名全称

Interferon regulatory factor 3

实验方案>

实验步骤

引用文献(0)>

清除