Anti-IRF3 Antibody (Clone#7B10)

筛选器： All WB ICC/IF FCM

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  Western blot analysis of IRF3 using anti-IRF3 antibody (M00165-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: mouse lung tissue lysates,
  Lane 3: mouse brain tissue lysates,
  Lane 4: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IRF3 antigen affinity purified monoclonal antibody (M00165-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IRF3 at approximately 50-55 kDa. The expected band size for IRF3 is at 47 kDa.

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  ICC/IF analysis of IRF3 using anti-IRF3 antibody (M00165-4).
  IRF3 was detected in an immunocytochemical section of RM-1 cells. The section was incubated with mouse anti-IRF3 Antibody (M00165-4) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of Hepa1-6 cells using anti-IRF3 antibody (M00165-4).
  Overlay histogram showing Hepa1-6 cells stained with M00165-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IRF3 Antibody (M00165-4) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of IRF3 using anti-IRF3 antibody (M00165-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: mouse lung tissue lysates,
  Lane 3: mouse brain tissue lysates,
  Lane 4: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-IRF3 antigen affinity purified monoclonal antibody (M00165-4) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IRF3 at approximately 50-55 kDa. The expected band size for IRF3 is at 47 kDa.

• 

  ICC/IF analysis of IRF3 using anti-IRF3 antibody (M00165-4).
  IRF3 was detected in an immunocytochemical section of RM-1 cells. The section was incubated with mouse anti-IRF3 Antibody (M00165-4) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of Hepa1-6 cells using anti-IRF3 antibody (M00165-4).
  Overlay histogram showing Hepa1-6 cells stained with M00165-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IRF3 Antibody (M00165-4) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.