Anti-IRF3 Antibody (Clone#OTI4D4)
送常用试剂

Mouse monoclonal antibody

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筛选器： All WB FCM IHC ICC/IF

MA00165-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-IRF3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IRF3 antibody, and then analyzed by flow cytometry.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, MA00165-1)
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Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY IRF3 using anti-IRF3 antibody. 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (1:500).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IRF3 Antibody (Clone#OTI4D4)

规格/价格

50μl|1280元
100μl|2180元
150μl|2800元

指标别称

interferon regulatory factor 3; IRF 3; IRF3

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

IRF3

克隆号

OTI4D4

宿主

Mouse

抗体亚型

IgG2a

免疫原

Full length human recombinant protein of human IRF3 (NP_001562) produced in HEK293T cell.

计算分子量

47 kDa

实际分子量

47 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

IRF3(interferon regulatory factor 3)?is a member of the interferon regulatory transcription factor(IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with?CREBBP. IRF3 plays an important role in the?innate immune system's response to?viral infection. Aggregated?MAVS?have been found to activate IRF3 dimerization. Although?IRF3?increased transcriptional activity from an ISRE-containing promoter, expression of?IRF3?as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase(CAT) reporter gene containing repeats of the Gal4-binding sites.?Translocation of?IRF3?was accompanied by an increase in serine and threonine phosphorylation.?The transcriptional activators CREBBP and EP300 coimmunoprecipitated with?IRF3?only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Uniprot ID

Q14653

文献引用格式

IRF3 Antibody (Clone#OTI4D4) (Boster Biological Technology, Wuhan, China. Catalog#MA00165-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interferon regulatory factor 3

蛋白名全称

Interferon regulatory factor 3

实验方案>

实验步骤

引用文献(0)>

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