Anti-IRF3 Antibody (Clone#OTI5D2)
送常用试剂

Mouse monoclonal antibody

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筛选器： All WB IHC

M00165-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (1:500).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-IRF3monoclonal antibody.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and IRF3-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-IRF3 monoclonal antibody M00165-1 (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
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Western blot analysis of extracts (35ug) from 4 different cell lines and human plasma by using anti-IRF3 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)
Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
all(18)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IRF3 Antibody (Clone#OTI5D2)

规格/价格

50μl|1280元
100μl|2180元
150μl|2800元

指标别称

interferon regulatory factor 3; IRF 3; IRF3

产品类型

Monoclonal

检验物种

human, monkey

应用范围

WB, IHC

基因名称

IRF3

克隆号

OTI5D2

宿主

Mouse

抗体亚型

IgG2b

免疫原

Full-length protein expressed in 293T cell transfected with human IRF3 expression vector

计算分子量

47 kDa

实际分子量

47.2 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

IRF3(interferon regulatory factor 3)?is a member of the interferon regulatory transcription factor(IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with?CREBBP. IRF3 plays an important role in the?innate immune system's response to?viral infection. Aggregated?MAVS?have been found to activate IRF3 dimerization. Although?IRF3?increased transcriptional activity from an ISRE-containing promoter, expression of?IRF3?as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase(CAT) reporter gene containing repeats of the Gal4-binding sites.?Translocation of?IRF3?was accompanied by an increase in serine and threonine phosphorylation.?The transcriptional activators CREBBP and EP300 coimmunoprecipitated with?IRF3?only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Uniprot ID

Q14653

文献引用格式

IRF3 Antibody (Clone#OTI5D2) (Boster Biological Technology, Wuhan, China. Catalog#M00165-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

interferon regulatory factor 3

蛋白名全称

Interferon regulatory factor 3

实验方案>

实验步骤

引用文献(0)>

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