Anti-IRF3(Phospho-S386) Antibody (Clone#IGO-9)
送常用试剂

Rabbit Monoclonal antibody

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筛选器： All WB ICC/IF

BM4844

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-P-IRF3 antibody (BM4844). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-P-IRF3 antigen affinity purified monoclonal antibody (BM4844) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for P-IRF3 at approximately 55 kDa. The expected band size for P-IRF3 is at 47 kDa.
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产品简介>

产品名称

Anti-IRF3(Phospho-S386) Antibody (Clone#IGO-9)

规格/价格

50μl|1280元
100μl|2180元
150μl|2800元

指标别称

interferon regulatory factor 3; IRF 3; IRF3

产品类型

Monoclonal

检验物种

human

应用范围

WB, ICC/IF

基因名称

IRF3

克隆号

IGO-9

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-IRF3 (S386)

计算分子量

47 kDa

实际分子量

50-55 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

IRF3(interferon regulatory factor 3) is a member of the interferon regulatory transcription factor(IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with°CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase(CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Uniprot ID

Q14653

文献引用格式

IRF3 (Phospho-S386) Antibody (Clone#IGO-9) (Boster Biological Technology, Wuhan, China. Catalog#BM4844)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Interferon regulatory factor 3

实验方案>

实验步骤

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