Anti-PRKAR1A Antibody (Clone#OTI6C7)

筛选器： All WB IHC ICC/IF

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and PRKAR1A-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A monoclonal antibody M00699, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([MA01692]) as a loading control.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PRKAR1A monoclonal antibody (1:200).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A (Cat# M00699).

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00699, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00699, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00699, Dilution 1:50)

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  Figure from citation: PRKAR1A IHC in a CNC-associated cardiac myxoma. A, A photomicrograph of the specimen at low power exhibits the robust reactivity of the adjacent normal myocardium with antibodies directed against PRKAR1A. B, A high-power photomicrograph exhibits the lack of reactivity seen in the neoplastic (myxoma) cells, although the intratumoral histiocytes are still strongly reactive. Dilution: 1:8000 View Citation

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00699, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00699, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00699, Dilution 1:50)

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  Anti-PRKAR1A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1A .

Anti-PRKAR1B Antibody (Clone#OTI2A3)

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  HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKAR1B antibody, and then analyzed by flow cytometry.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M07227)

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  Flow cytometric Analysis of Hela cells, using anti-PRKAR1B antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M07227)

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  Anti-PRKAR1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1B .

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  Flow cytometric Analysis of Jurkat cells, using anti-PRKAR1B antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1B (Cat# M07227).

Anti-p130 Cas/BCAR1 Antibody (Clone#OTI3A11)

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00960-3)

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  HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCAR1 antibody, and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

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  Anti-BCAR1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1 .

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  Flow cytometric Analysis of Jurkat cells, using anti-BCAR1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1.

Anti-EDG2/LPAR1 Antibody (Clone#OTI1G6)

筛选器： All WB ICC/IF FCM

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LPAR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPAR1(Cat# M02782-1).

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  Anti-LPAR1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LPAR1 .

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  HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LPAR1 antibody, and then analyzed by flow cytometry.

Anti-CA1 Antibody (Clone#2B5)

筛选器： All WB IHC

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  Western blot analysis of CA1 using anti-CA1 antibody (M00170-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human K562 whole cell lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CA1 antigen affinity purified monoclonal antibody (M00170-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CA1 at approximately 29 kDa. The expected band size for CA1 is at 29 kDa.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-2).
  CA1 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-2).
  CA1 was detected in a paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-2).
  CA1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-2).
  CA1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-2).
  CA1 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-2).
  CA1 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

Anti-IQGAP1 Antibody (Clone#OTI6E10)

筛选器： All IHC WB

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IQGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01603-2) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IQGAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, M01603-2) (1:2000)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IQGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IQGAP1 (1:2000).

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  Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-IQGAP1 monoclonal antibody (1:500).

Anti-MARK3 Antibody (Clone#OTI2A4)

筛选器： All WB

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3.

Anti-PIN4 Antibody (Clone#OTI7H2)

筛选器： All IHC WB

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PIN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, M05181) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PIN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, M05181) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PIN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, M05181) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PIN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, M05181) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PIN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, M05181) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PIN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, M05181) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, M05181) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PIN4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, M05181) (1:150)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIN4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIN4 (Cat# M05181)(1:2000).

Anti-CA1 Antibody (Clone#4D5)

筛选器： All WB IHC FCM

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  Western blot analysis of CA1 using anti-CA1 antibody (M00170-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human K562 whole cell lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CA1 antigen affinity purified monoclonal antibody (M00170-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CA1 at approximately 29 kDa. The expected band size for CA1 is at 29 kDa.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-1).
  CA1 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-1).
  CA1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-1).
  CA1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-1).
  CA1 was detected in a paraffin-embedded section of human prostate cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-1).
  CA1 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-1).
  CA1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CA1 using anti-CA1 antibody (M00170-1).
  CA1 was detected in a paraffin-embedded section of human prostate cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CA1 Antibody (M00170-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of SiHa cells using anti-CA1 antibody (M00170-1).
  Overlay histogram showing SiHa cells stained with M00170-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CA1 Antibody (M00170-1) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-NR1I3 Antibody (Clone#OTI1B2)

筛选器： All IHC WB

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NR1I3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M02858-1)

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-NR1I3 monoclonal antibody (1:200).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1I3.

Anti-Calcium Sensing Receptor/CASR Antibody (Clone#11E9)

筛选器： All WB FCM

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  Western blot analysis of anti-Calcium Sensing Receptor/CASR antibody (M00574). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysate,
  Lane 2: human A549 whole cell lysate,
  Lane 3: human 22RV1 whole cell lysate,
  Lane 4: human HepG2 whole cell lysate,
  Lane 5: human Caco-2 whole cell lysate.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Calcium Sensing Receptor/CASR antigen affinity purified monoclonal antibody (M00574) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calcium Sensing Receptor/CASR at approximately 130 kDa. The expected band size for Calcium Sensing Receptor/CASR is at 121 kDa.

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  Flow Cytometry analysis of Neuro-2a cells using anti-Calcium Sensing Receptor/CASR antibody (M00574).
  Overlay histogram showing Neuro-2a cells stained with M00574 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Calcium Sensing Receptor/CASR Antibody (M00574) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.