
Western blot analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (BA0644). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat skeletal muscle tissue lysates,
Lane 3: Rat ovary tissue lysates,
Lane 4: Rat lung tissue lysates,
Lane 5: MCF-7 whole cell lysates,
Lane 6: SMMC whole cell lysates,
Lane 7: A549 whole cell lysates,
Lane 8: JURKAT whole cell lysates,
Lane 9: SGC whole cell lysates,
Lane 10: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Annexin V/ANXA5 antigen affinity purified polyclonal antibody (BA0644) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Annexin V/ANXA5 at approximately 36 kDa. The expected band size for Annexin V/ANXA5 is at 36 kDa.