BSA标准品(2mg/ml)

BSA TBS-T(含有0.05％Tween-20)缓冲系统封闭液

5%BSA封闭液

BSA

Anti-BSAP/PAX5 Antibody (Clone#ICD-16)

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of PAX5 expression in Ramos cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human tonsil, using PAX5 Antibody.

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  Immunofluorescent analysis using the Antibody.

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  Immunofluorescent analysis of Ramos cells, using PAX5 Antibody.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

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Anti-BSAP/PAX5 Antibody

筛选器： All WB IHC ICC/IF ELISA

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  Western blot analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Raji whole cell lysates,
  Lane 2: Ramos whole cell lysates,
  Lane 3: Daudi whole cell lysates,
  Lane 4: Jurkat whole cell lysates,
  Lane 5: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BSAP/PAX5 antigen affinity purified polyclonal antibody (A00669-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSAP/PAX5 at approximately 45 kDa. The expected band size for BSAP/PAX5 is at 42 kDa.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-1).
  BSAP/PAX5 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSAP/PAX5 Antibody (A00669-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-1).
  BSAP/PAX5 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSAP/PAX5 Antibody (A00669-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-1).
  BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSAP/PAX5 Antibody (A00669-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of PAX5 using anti-PAX5 antibody (A00669-1).
  PAX5 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-PAX5 Antibody (A00669-1) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody.

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  IF analysis of PAX5 using anti-PAX5 antibody (A00669-1).
  PAX5 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-PAX5 Antibody (A00669-1) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody.

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Anti-BSAP/PAX5 Antibody

筛选器： All WB IHC FCM ELISA

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  Western blot analysis of anti-BSAP/PAX5 antibody (A00669-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Ramos whole cell lysates,
  Lane 2: human Daudi whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BSAP/PAX5 antigen affinity purified polyclonal antibody (A00669-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSAP/PAX5 at approximately 45 kDa. The expected band size for BSAP/PAX5 is at 42,45 kDa.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-2).
  BSAP/PAX5 was detected in a paraffin-embedded section of human lymphoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-2).
  BSAP/PAX5 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (A00669-2).
  BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of U2OS cells using anti-BSAP/PAX5 antibody (A00669-2).
  Overlay histogram showing U2OS cells stained with A00669-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BSAP/PAX5 Antibody (A00669-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-BSAP/PAX5 Antibody (Clone#OTI3A7)

筛选器： All IHC WB

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00669-3)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00669-3) (1:200)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00669-3)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00669-3) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00669-3)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, M00669-3) (1:200)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00669-3)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAX5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAX5(Cat# M00669-3).

Anti-BSAP/PAX5 Antibody (Clone#6I4)

筛选器： All WB IHC ICC/IF

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
  BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of PAX5 using anti- PAX5 antibody (M00669-2)
  PAX5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL mouse anti- PAX5 Antibody (M00669-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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  Western blot analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Raji whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BSAP/PAX5 antigen affinity purified monoclonal antibody (M00669-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSAP/PAX5 at approximately 45 kDa. The expected band size for BSAP/PAX5 is at 42 kDa.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
  BSAP/PAX5 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
  BSAP/PAX5 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BSAP/PAX5 using anti-BSAP/PAX5 antibody (M00669-2).
  BSAP/PAX5 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-BSAP/PAX5 Antibody (M00669-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

Anti-JNK1/2/3 Antibody (Clone#DFI-13)

筛选器： All WB ICC/IF IP

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  Western blot analysis of anti-JNK1/2/3 antibody (BM4329). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human U87 whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: mouse Neuro-2a whole cell lysates,
  Lane 7: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-JNK1/2/3 antigen affinity purified monoclonal antibody (BM4329) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for JNK1/2/3 at approximately 40, 48 kDa. The expected band size for JNK1/2/3 is at 48 kDa.

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Anti-MAPK8/10 Antibody (Clone#ICC-13)

筛选器： All WB IP

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  Western blot analysis of JNK1/JNK3 expression in HeLa cell lysate.

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Anti-Hepatitis B Virus Antibody

筛选器： All IHC WB

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  IHC analysis of Hepatitis B Virus using anti-Hepatitis B Virus antibody (A30379).
  Hepatitis B Virus was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Hepatitis B Virus Antibody (A30379) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Hepatitis B Virus using anti-Hepatitis B Virus antibody (A30379).
  Hepatitis B Virus was detected in a paraffin-embedded section of human hepatitis B tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Hepatitis B Virus Antibody (A30379) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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Anti-MKL1/MRTFA Antibody

筛选器： All WB ICC/IF ELISA

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  Western blot analysis of MKL1/MRTFA using anti-MKL1/MRTFA antibody (A32468-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MKL1/MRTFA antigen affinity purified polyclonal antibody (A32468-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MKL1/MRTFA at approximately 145 kDa. The expected band size for MKL1/MRTFA is at 99 kDa.

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  IF analysis of MKL1/MRTFA using anti-MKL1/MRTFA antibody (A32468-2).
  MKL1/MRTFA was detected in an immunocytochemical section of SiHa cells. The section was incubated with rabbit anti-MKL1/MRTFA Antibody (A32468-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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Anti-JNK1/2/3 (Phospho-T183+T183+T221) Antibody (Clone#EAG-13)

筛选器： All WB IHC ICC/IF IP FCM

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  Western blot analysis of JNK1/2/3 phosphorylation expression in NIH/3T3 cell lysate treated with Anisomycin.

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  All lanes use the Antibody for 1 hour at room temperature.

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  IHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (BM4380) .
  P-JNK1/2/3 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-P-JNK1/2/3 Antibody (BM4380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (BM4380) .
  P-JNK1/2/3 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-P-JNK1/2/3 Antibody (BM4380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (BM4380) .
  P-JNK1/2/3 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-P-JNK1/2/3 Antibody (BM4380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of P-JNK1/2/3 using anti-P-JNK1/2/3 antibody (BM4380) .
  P-JNK1/2/3 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-P-JNK1/2/3 Antibody (BM4380) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  Immunofluorescent analysis using the Antibody.

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  Immunofluorescent analysis using the Antibody.

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  Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using Phospho-JNK1/2/3 (T183+T183+T221) Antibody.

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Anti-Hepatitis B Virus Antibody (Clone#OTIR2G9)

筛选器： All WB

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  Western blot analysis of recombinant fusion protein of SARS-CoV-2 spike RBD protein and virion surface domain of membrane protein ( 0.05ug) by using anti-SARS-CoV-2 spike protein antibody (Cat# M30379-1).

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  Western blot analysis of recombinant SARS-CoV-2 spike-RBD protein ( 0.02ug) by using anti-SARS-CoV-2 spike protein antibody (Cat# M30379-1).

Anti-MAPK10 Antibody (Clone#HGC-13)

筛选器： All WB ICC/IF FCM

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  Western blot analysis of anti-MAPK10 antibody (BM4743). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAPK10 antigen affinity purified monoclonal antibody (BM4743) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MAPK10 at approximately 50, 53 kDa. The expected band size for MAPK10 is at 53kDa.

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  Immunofluorescent analysis using the Antibody.

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  Immunofluorescent analysis using the Antibody.

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Anti-MKL1/MRTFA Antibody (Clone#OTI2B9)

筛选器： All WB

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKL1.