Anti-AKR1C3 Antibody

筛选器： All WB FCM

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  Western blot analysis of AKR1C3 using anti-AKR1C3 antibody (A01820-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HCCP whole cell lysates,
  Lane 2: A549 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKR1C3 antigen affinity purified polyclonal antibody (A01820-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKR1C3 at approximately 37 kDa. The expected band size for AKR1C3 is at 37 kDa.

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  Flow Cytometry analysis of HepG2 cells using anti-AKR1C3 antibody (A01820-1).
  Overlay histogram showing HepG2 cells stained with A01820-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR1C3 Antibody (A01820-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-CDC7 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of CDC7 using anti-CDC7 antibody (A01190-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Jurkat whole cell lysates,
  Lane 2: MCF-7 whole cell lysates,
  Lane 3: Hela whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDC7 antigen affinity purified polyclonal antibody (A01190-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDC7 at approximately 64 kDa. The expected band size for CDC7 is at 64 kDa.

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  Flow Cytometry analysis of A431 cells using anti-CDC7 antibody (A01190-3).
  Overlay histogram showing A431 cells stained with A01190-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC7 Antibody (A01190-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-CHAT Antibody

筛选器： All WB IHC ELISA

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  Western blot analysis of CHAT using anti-CHAT antibody (A01192-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CHAT antigen affinity purified polyclonal antibody (A01192-5) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CHAT at approximately 83 kDa. The expected band size for CHAT is at 83 kDa.

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  IHC analysis of CHAT using anti-CHAT antibody (A01192-5).
  CHAT was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CHAT Antibody (A01192-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of CHAT using anti-CHAT antibody (A01192-5).
  CHAT was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CHAT Antibody (A01192-5) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Anti-CHAT Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Western blot analysis of CHAT using anti-CHAT antibody (A01192-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta whole cell lysates,
  Lane 2: human U-87MG tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CHAT antigen affinity purified polyclonal antibody (A01192-4) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CHAT at approximately 83 kDa. The expected band size for CHAT is at 83 kDa.

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  IHC analysis of CHAT using anti-CHAT antibody (A01192-4).
  CHAT was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CHAT Antibody (A01192-4) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of CHAT using anti-CHAT antibody (A01192-4).
  CHAT was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-CHAT Antibody (A01192-4) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of SiHa cells using anti-CHAT antibody (A01192-4).
  Overlay histogram showing SiHa cells stained with A01192-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHAT Antibody (A01192-4) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Cytoglobin/CYGB Antibody

筛选器： All WB IHC

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  Western blot analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat small intestine tissue lysates,
  Lane 2: mouse small intestine tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytoglobin/CYGB antigen affinity purified polyclonal antibody (A01197) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytoglobin/CYGB at approximately 21 kDa. The expected band size for Cytoglobin/CYGB is at 21 kDa.

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  IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
  Cytoglobin/CYGB was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
  Cytoglobin/CYGB was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
  Cytoglobin/CYGB was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
  Cytoglobin/CYGB was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Cytoglobin/CYGB using anti-Cytoglobin/CYGB antibody (A01197).
  Cytoglobin/CYGB was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Cytoglobin/CYGB Antibody (A01197) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Anti-HLA-DRA Antibody

筛选器： All WB IHC FCM ELISA

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  Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (A01195). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Raji whole cell lysates,
  Lane 2: human Daudi whole cell lysates,
  Lane 3: human Ramos whole cell lysates,
  Lane 4: human HMy2,CIR whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HLA-DRA antigen affinity purified polyclonal antibody (A01195) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HLA-DRA at approximately 36 kDa. The expected band size for HLA-DRA is at 29 kDa.

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  IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
  HLA-DRA was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
  HLA-DRA was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
  HLA-DRA was detected in a paraffin-embedded section of human duodenal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
  HLA-DRA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of HLA-DRA using anti-HLA-DRA antibody (A01195).
  HLA-DRA was detected in a paraffin-embedded section of human seminoma testis tissue. The tissue section was incubated with rabbit anti-HLA-DRA Antibody (A01195) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  Flow cytometry analysis of h_PBMC cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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Anti-STS Antibody

筛选器： All WB IHC FCM ELISA

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  Western blot analysis of STS using anti-STS antibody (A01198-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STS antigen affinity purified polyclonal antibody (A01198-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STS at approximately 65 kDa. The expected band size for STS is at 65 kDa.

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  IHC analysis of STS using anti-STS antibody (A01198-2).
  STS was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-STS Antibody (A01198-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of U87 cells using anti-STS antibody (A01198-2).
  Overlay histogram showing U87 cells stained with A01198-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STS Antibody (A01198-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-STS Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of STS using anti-STS antibody (A01198-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: human PANC-1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STS antigen affinity purified polyclonal antibody (A01198-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STS at approximately 65 kDa. The expected band size for STS is at 65 kDa.

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  Flow Cytometry analysis of Hela cells using anti-STS antibody (A01198-1).
  Overlay histogram showing Hela cells stained with A01198-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-STS Antibody (A01198-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-VCAM1 Antibody

筛选器： All WB ELISA(Cap)

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  Western blot analysis of VCAM1 using anti-VCAM1 antibody (A01199-1).
  Lane 1: recombinant human VCAM1 protein 1ng.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody (A01199-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VCAM1 at approximately 85-110 kDa.

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Anti-VCAM1 Antibody

筛选器： All WB IHC ELISA(Cap)

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  Western blot analysis of VCAM1 using anti-VCAM1 antibody (A01199). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse spleen tissue lysates,
  Lane 2: mouse lung tissue lysates,
  Lane 3: rat spleen tissue lysates,
  Lane 4: rat lung tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody (A01199) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VCAM1 at approximately 85-110 kDa. The expected band size for VCAM1 is at 81 kDa.

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  IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199).
  VCAM1 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VCAM1 Antibody (A01199) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199).
  VCAM1 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VCAM1 Antibody (A01199) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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Anti-MAPKAPK2 Antibody

筛选器： All WB IHC ICC/IF ELISA