Anti-RBM41 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of RBM41 using anti-RBM41 antibody (A14311-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: SGC-7901 whole cell lysates,
  Lane 2: A549 whole cell lysates,
  Lane 3: HEK293 whole cell lysates,
  Lane 4: rat liver tissue lysates,
  Lane 5: mouse liver tissue lysates,
  Lane 6: HEPA1-6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RBM41 antigen affinity purified polyclonal antibody (A14311-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RBM41 at approximately 47 kDa. The expected band size for RBM41 is at 47 kDa.

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  Flow Cytometry analysis of Jurkat cells using anti-RBM41 antibody (A14311-1).
  Overlay histogram showing Jurkat cells stained with A14311-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM41 Antibody (A14311-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-RBM42 Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Western blot analysis of RBM42 using anti-RBM42 antibody (A14229-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human 293T whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RBM42 antigen affinity purified polyclonal antibody (A14229-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RBM42 at approximately 65 kDa. The expected band size for RBM42 is at 50 kDa.

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  IHC analysis of RBM42 using anti-RBM42 antibody (A14229-1) .
  RBM42 was detected in a paraffin-embedded section of human cervix squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-RBM42 Antibody (A14229-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of RBM42 using anti-RBM42 antibody (A14229-1) .
  RBM42 was detected in a paraffin-embedded section of human cervix squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-RBM42 Antibody (A14229-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of RBM42 using anti-RBM42 antibody (A14229-1) .
  RBM42 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-RBM42 Antibody (A14229-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of RBM42 using anti-RBM42 antibody (A14229-1) .
  RBM42 was detected in a paraffin-embedded section of mouse stomach tissue. The tissue section was incubated with rabbit anti-RBM42 Antibody (A14229-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of RBM42 using anti-RBM42 antibody (A14229-1) .
  RBM42 was detected in a paraffin-embedded section of rat stomach tissue. The tissue section was incubated with rabbit anti-RBM42 Antibody (A14229-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of RBM42 using anti-RBM42 antibody (A14229-1) and anti-Beta Tubulin antibody (M01857-3).
  RBM42 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-RBM42 Antibody (A14229-1) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green)(Catalog#BA1101) were used as secondary antibody.

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  Flow Cytometry analysis of 293T cells using anti-RBM42 antibody (A14229-1).
  Overlay histogram showing 293T cells stained with A14229-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM42 Antibody (A14229-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Anti-RBM47 Antibody

筛选器： All WB FCM ICC/IF ELISA

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  Western blot analysis of RBM47 using anti-RBM47 antibody (A13214-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human A549 whole cell lysates,
  Lane 2: human CACO-2 whole cell lysates,
  Lane 3: human A431 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RBM47 antigen affinity purified polyclonal antibody (A13214-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RBM47 at approximately 64 kDa. The expected band size for RBM47 is at 64 kDa.

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  Flow Cytometry analysis of A431 cells using anti-RBM47 antibody (A13214-1).
  Overlay histogram showing A431 cells stained with A13214-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM47 Antibody (A13214-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  IF analysis of RBM47 using anti-RBM47 antibody (A13214-1).
  RBM47 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-RBM47 Antibody (A13214-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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Anti-RBM4 Antibody

筛选器： All WB IHC IP ELISA

Anti-14-3-3 alpha/beta Antibody (Clone#HHA-25)

筛选器： All WB IHC ICC/IF IP FCM

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  Western blot analysis of anti-14-3-3 alpha/beta antibody (BM4752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Caco-2 whole cell lysates,
  Lane 3: human SiHa whole cell lysates,
  Lane 4: human Hacat whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat lung tissue lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse lung tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 alpha/beta antigen affinity purified monoclonal antibody (BM4752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 alpha/beta at approximately 28 kDa. The expected band size for 14-3-3 alpha/beta is at 28 kDa.

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Anti-53BP2/TP53BP2 Antibody (Clone#AOEA-20)

筛选器： All WB IHC ICC/IF

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  Western blot analysis of ASPP2 expression in MCF7 cell lysate.

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Anti-ACACA/B Antibody (Clone#EDI-1)

筛选器： All WB IHC

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  Western blot analysis of anti-ACC1/ACACA antibody (BM4414). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: rat liver tissue lysates,
  Lane 3: mouse liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACC1/ACACA antigen affinity purified monoclonal antibody (BM4414) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACC1/ACACA at approximately 266 kDa. The expected band size for ACC1/ACACA is at 266 kDa.

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  Immunohistochemical analysis of paraffin-embedded human kidney, using Acetyl-CoA Carboxylase Antibody.

Anti-ACE2 Antibody (Clone#GEE-1)

筛选器： All WB IHC ICC/IF IP

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  Western blot analysis of ACE2 expression in Human kidney lysate.

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Anti-ADIPOR1 Antibody (Clone#FIH-1)

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of ADIPOR1 expression in Human heart lysate.

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  Immunohistochemical analysis of paraffin-embedded human kidney, using ADIPOR1 Antibody.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

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Anti-AFP Antibody (Clone#AAOO-1)

筛选器： All WB IHC ICC/IF IP FCM

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  Western blot analysis of AFP expression in HepG2 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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Anti-AGO2 Antibody (Clone#AODE-1)

筛选器： All WB IHC ICC/IF IP FCM

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  Western blot analysis of anti-AGO2 antibody (BM4920). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGO2 antigen affinity purified monoclonal antibody (BM4920) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGO2 at approximately 97 kDa. The expected band size for AGO2 is at 97 kDa.

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Anti-AGTR1 Antibody (Clone#AOGD-1)

筛选器： All WB

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  Western blot analysis of AGTR1 expression in (1) HeLa cell lysate; (2) PC-12 cell lysate.

Anti-AGTR2 Antibody (Clone#FIO-1)

筛选器： All WB IP

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  Western blot analysis of anti-AGTR2 antibody (BM4557). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human SH-SY5Y whole cell lysates,
  Lane 3: human Caco-2 whole cell lysates,
  Lane 4: rat lung tissue lysates,
  Lane 5: mouse lung tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGTR2 antigen affinity purified monoclonal antibody (BM4557) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGTR2 at approximately 45 kDa. The expected band size for AGTR2 is at 41 kDa.

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Anti-AHR Antibody (Clone#AOHI-1)

筛选器： All WB ICC/IF

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  Western blot analysis of anti-AHR antibody (BM4965). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human RT4 whole cell lysates,
  Lane 2: human U-87MG whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AHR antigen affinity purified monoclonal antibody (BM4965) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AHR at approximately 96, 105 kDa. The expected band size for AHR is at 96 kDa.

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Anti-AKT1 Antibody (Clone#EBG-1)

筛选器： All WB IHC ICC/IF IP FCM

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  Western blot analysis of anti- AKT1 antibody (BM4390). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: HEPG2 whole cell lysates,
  Lane 2: A549 whole cell lysates,
  Lane 3: HELA whole cell lysates,
  Lane 4: Raji whole cell lysates,
  Lane 5: MCF-7 whole cell lysates,
  Lane 6: SIHA whole cell lysates,
  Lane 7: 293T whole cell lysates.
  Use rabbit Anti-AKT1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AKT1 at approximately 60KD. The expected band size for AKT1 is at 56KD.

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  Western blot analysis of anti- AKT1 antibody (BM4390). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: C6 whole cell lysates,
  Lane 4: RH35 whole cell lysates,
  Lane 5:mouse liver tissue lysates,
  Lane 6: mouse brain tissue lysates,
  Lane 7: Neuro-2a whole cell lysates.
  Lane 8: HEPA1-6 whole cell lysates.
  Use rabbit Anti-AKT1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for AKT1 at approximately 60KD. The expected band size for AKT1 is at 56KD.

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Anti-AKT1/2 Antibody (Clone#17A25)

筛选器： All WB IHC ICC/IF FCM

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  Western blot analysis of AKT1/2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

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Anti-AKT1/2/3 Antibody (Clone#17A20)

筛选器： All WB IHC ICC/IF IP FCM

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  Western blot analysis of anti-AKT1/2/3 antibody (BM4400). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: human 293T whole cell lysates,
  Lane 4: human U2OS whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat heart tissue lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1/2/3 antigen affinity purified monoclonal antibody (BM4400) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT1/2/3 at approximately 60 kDa. The expected band size for AKT1/2/3 is at 60 kDa.

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  Immunohistochemical analysis of paraffin-embedded human testis, using Akt(pan)1/2/3 Antibody.

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  Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody.

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  Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody.

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  Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using the Antibody.

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  Immunofluorescent analysis of MCF-7 cells, using Akt(pan)1/2/3 Antibody.

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  Immunoprecipitate (IP) analysis using the Antibody. (wb)

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Anti-ALDH1A1 Antibody (Clone#AHA-1)

筛选器： All WB IHC ICC/IF IP FCM

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  Western blot analysis of anti-ALDH1A1 antibody (BM4034). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: rat kidney tissue lysates,
  Lane 4: mouse liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALDH1A1 antigen affinity purified monoclonal antibody (BM4034) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH1A1 at approximately 50 kDa. The expected band size for ALDH1A1 is at 55 kDa.

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  Immunohistochemical analysis of paraffin-embedded human liver cancer, using ALDH1A1 Antibody.

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Anti-ALPL Antibody (Clone#DBF-1)

筛选器： All WB IHC ICC/IF IP FCM

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  Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.

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  Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody .

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  Western blot analysis of anti-ALPL antibody (BM4284). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human K562 whole cell lysates,
  Lane 5: rat liver tissue lysates,
  Lane 6: rat spleen tissue lysates,
  Lane 7: mouse liver tissue lysates,
  Lane 8: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALPL antigen affinity purified monoclonal antibody (BM4284) at a dilution of 1:2000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALPL at approximately 80 kDa. The expected band size for ALPL is at 57 kDa.

Anti-AMPK Alpha 1/PRKAA1 Antibody (Clone#CDD-16)

筛选器： All WB IHC ICC/IF IP FCM

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  Western blot analysis of anti-AMPK Alpha 1/PRKAA1 antibody (BM4202). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human U-87 MG whole cell lysates,
  Lane 3: human PC-3 whole cell lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: rat RH-35 whole cell lysates,
  Lane 6: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AMPK Alpha 1/PRKAA1 antigen affinity purified monoclonal antibody (BM4202) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AMPK Alpha 1/PRKAA1 at approximately 64 kDa. The expected band size for AMPK Alpha 1/PRKAA1 is at 64 kDa.

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  Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody.

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  Immunohistochemical analysis of paraffin-embedded Human tonsil, using the Antibody.

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  Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody.

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  Immunohistochemical analysis of paraffin-embedded human kidney, using AMPK alpha 1 Antibody.

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  Immunofluorescent analysis of Hela cells, using AMPK alpha 1 Antibody.

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