Western blot analysis of Osteoglycin/OGN using anti-Osteoglycin/OGN antibody (A07061-1).
Lane 1: recombinant mouse OGN protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Osteoglycin/OGN antigen affinity purified polyclonal antibody (A07061-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Osteoglycin/OGN at approximately 34 kDa.

Western blot analysis of BMP7 using anti-BMP7 antibody (BA0665-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BMP7 antigen affinity purified polyclonal antibody (BA0665-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BMP7 at approximately 49 kDa. The expected band size for BMP7 is at 49 kDa.

Western blot analysis of CD44 using anti-CD44 antibody (A00052-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PANC-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (A00052-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD44 at approximately 85 kDa. The expected band size for CD44 is at 82 kDa.

IHC analysis of CD44 using anti-CD44 antibody (A00052-2) .
CD44 was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD44 using anti-CD44 antibody (A00052-2) .
CD44 was detected in a paraffin-embedded section of mouse lymphaden tissue. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD44 using anti-CD44 antibody (A00052-2) .
CD44 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD44 using anti-CD44 antibody (A00052-2) .
CD44 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IF analysis of CD44 using anti-CD44 antibody (A00052-2).
CD44 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-CD44 Antibody (A00052-2) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Flow Cytometry analysis of MCF-7 cells using anti-CD44 antibody (A00052-2).
Overlay histogram showing MCF-7 cells stained with A00052-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD44 Antibody (A00052-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of anti- CD44 antibody (PB9333). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human PC-12 whole cell lysates,
Lane 4: human C6 whole cell lysates.
Use rabbit anti- CD44 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CD44 at approximately 82KD. The expected band size for CD44 is at 82KD.

IHC analysis of CD44 using anti-CD44 antibody (PB9333).
CD44 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD44 Antibody (PB9333) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IF analysis of CD44 using anti-CD44 antibody (PB9333)
CD44 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of CD44 using anti-CD44 antibody (PB9333)
CD44 was detected in paraffin-embedded section of rat lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of CD44 using anti-CD44 antibody (A00052). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat small intestine tissue lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (A00052) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD44 at approximately 82 kDa. The expected band size for CD44 is at 82 kDa.

IHC analysis of CD44 using anti-CD44 antibody (A00052).
CD44 was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD44 using anti-CD44 antibody (A00052).
CD44 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD44 using anti-CD44 antibody (A00052).
CD44 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of CD44 using anti-CD44 antibody (A00052).
CD44 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD44 Antibody (A00052) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IF analysis of CD44 using anti-CD44 antibody (A00052)CD44 was detected in paraffin-embedded section of mouse lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD44 Antibody (A00052) overnight at 4°C. DyLight 488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of CD44 using anti-CD44 antibody (A00052)CD44 was detected in paraffin-embedded section of mouse lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD44 Antibody (A00052) overnight at 4°C. DyLight 488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Flow Cytometry analysis of Jurkat cells using anti-CD44 antibody (A00052).
Overlay histogram showing Jurkat cells stained with A00052 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD44 Antibody (A00052) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of Decorin/DCN using anti-Decorin/DCN antibody (PB9174).
Lane 1: recombinant human Decorin protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Decorin/DCN antigen affinity purified polyclonal antibody (PB9174) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Decorin/DCN at approximately 38 kDa.

Western blot analysis of Decorin/DCN using anti-Decorin/DCN antibody (PB9174). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat lung tissue lysates,
Lane 5: MCF-7 whole cell lysates,
Lane 6: SW620 whole cell lysates,
Lane 7: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Decorin/DCN antigen affinity purified polyclonal antibody (PB9174) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Decorin/DCN at approximately 40 kDa. The expected band size for Decorin/DCN is at 40 kDa.

IHC analysis of Decorin/DCN using anti-Decorin/DCN antibody (PB9174).
Decorin/DCN was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Decorin/DCN Antibody (PB9174) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of Decorin/DCN using anti-Decorin/DCN antibody (BA0798-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Liver tissue lysates,
Lane 2: Rat kidney tissue lysates,
Lane 3: Rat Spleen tissue lysates,
Lane 4: Rat Lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Decorin/DCN antigen affinity purified polyclonal antibody (BA0798-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Decorin/DCN at approximately 40 kDa. The expected band size for Decorin/DCN is at 40 kDa.

Western blot analysis of Decorin/DCN using anti-Decorin/DCN antibody (BA0798). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates,
Lane 2: Rat Spleen tissue lysates,
Lane 3: Rat Lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Decorin/DCN antigen affinity purified polyclonal antibody (BA0798) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Decorin/DCN at approximately 40 kDa. The expected band size for Decorin/DCN is at 40 kDa.

IHC analysis of Decorin/DCN using anti-Decorin/DCN antibody (BA0798).
Decorin/DCN was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Decorin/DCN Antibody (BA0798) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HAPLN1 antigen affinity purified polyclonal antibody (A05980-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HAPLN1 at approximately 40,48 kDa. The expected band size for HAPLN1 is at 40,48 kDa.

IHC analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3) .
HAPLN1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-HAPLN1 Antibody (A05980-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of HSPG2 using anti-HSPG2 antibody (PB9277). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSPG2 antigen affinity purified polyclonal antibody (PB9277) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HSPG2 at approximately 469 kDa. The expected band size for HSPG2 is at 469 kDa.

IHC analysis of HSPG2 using anti-HSPG2 antibody (PB9277).
HSPG2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-HSPG2 Antibody (PB9277) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of HSPG2 using anti-HSPG2 antibody (BA3247-1).
HSPG2 was detected in a paraffin-embedded section of rat ovary tissue. The tissue section was incubated with rabbit anti-HSPG2 Antibody (BA3247-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of anti-IMPG2 antibody (A10288-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IMPG2 antigen affinity purified polyclonal antibody (A10288-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IMPG2 at approximately 150 kDa. The expected band size for IMPG2 is at 139 kDa.

IHC analysis of IMPG2 using anti-IMPG2 antibody (A10288-1).
IMPG2 was detected in a paraffin-embedded section of rat retina tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of anti-Lumican/LUM antibody (A01034-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lumican/LUM antigen affinity purified polyclonal antibody (A01034-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lumican/LUM at approximately 70 kDa. The expected band size for Lumican/LUM is at 38 kDa.

IHC analysis of Lumican/LUM using anti-Lumican/LUM antibody (A01034-2).
Lumican/LUM was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lumican/LUM using anti-Lumican/LUM antibody (A01034-2).
Lumican/LUM was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lumican/LUM using anti-Lumican/LUM antibody (A01034-2).
Lumican/LUM was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

IHC analysis of Lumican/LUM using anti-Lumican/LUM antibody (A01034-2).
Lumican/LUM was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Flow Cytometry analysis of SH-SY5Y cells using anti-Lumican/LUM antibody (A01034-2).
Overlay histogram showing SH-SY5Y cells stained with A01034-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Lumican/LUM Antibody (A01034-2, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of NG2/CSPG4 using anti-NG2/CSPG4 antibody (A03394-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A375 tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NG2/CSPG4 antigen affinity purified polyclonal antibody (A03394-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NG2/CSPG4 at approximately 280,310 kDa. The expected band size for NG2/CSPG4 is at 251 kDa.

Western blot analysis of anti-OMD antibody (A05351-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: mouse bone tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OMD antigen affinity purified polyclonal antibody (A05351-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for OMD at approximately 60 kDa. The expected band size for OMD is at 49 kDa.

Flow Cytometry analysis of U2OS cells using anti-OMD antibody (A05351-1).
Overlay histogram showing U2OS cells stained with A05351-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OMD Antibody (A05351-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Western blot analysis of POMGNT2 using anti-POMGNT2 antibody (A11987-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POMGNT2 antigen affinity purified polyclonal antibody (A11987-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for POMGNT2 at approximately 75 kDa. The expected band size for POMGNT2 is at 67 kDa.

Western blot analysis of PRG2 using anti-PRG2 antibody (BA3806). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse HEPA1/6 whole cell lysates,
Lane 2: human CEM whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRG2 antigen affinity purified polyclonal antibody (BA3806) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PRG2 at approximately 24 kDa. The expected band size for PRG2 is at 24,25 kDa.

IHC analysis of PRG2 using anti-PRG2 antibody (BA3806).
PRG2 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PRG2 Antibody (BA3806) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Western blot analysis of SPOCK2 using anti-SPOCK2 antibody (A08811-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SPOCK2 antigen affinity purified polyclonal antibody (A08811-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SPOCK2 at approximately 55 kDa. The expected band size for SPOCK2 is at 47 kDa.

Western blot analysis of anti- SPOCK3 antibody (A03151-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human RT4 whole cell lysates.
Use rabbit anti- SPOCK3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SPOCK3 at approximately 38KD. The expected band size for SPOCK3 is at 49KD.

IF analysis of SPOCK3 using anti-SPOCK3 antibody (A03151-1).
SPOCK3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-SPOCK3 Antibody (A03151-1) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).