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Western blot analysis of anti-ASK1/MAP3K5 antibody (BM4220). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ASK1/MAP3K5 antigen affinity purified monoclonal antibody (BM4220) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ASK1/MAP3K5 at approximately 155 kDa. The expected band size for ASK1/MAP3K5 is at 155 kDa.
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IHC analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (BM4220) .
ASK1/MAP3K5 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ASK1/MAP3K5 Antibody (BM4220) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (BM4220) .
ASK1/MAP3K5 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ASK1/MAP3K5 Antibody (BM4220) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (BM4220) .
ASK1/MAP3K5 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-ASK1/MAP3K5 Antibody (BM4220) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (BM4220) .
ASK1/MAP3K5 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-ASK1/MAP3K5 Antibody (BM4220) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (BM4220) .
ASK1/MAP3K5 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. The tissue section was incubated with rabbit anti-ASK1/MAP3K5 Antibody (BM4220) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (BM4220) .
ASK1/MAP3K5 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. The tissue section was incubated with rabbit anti-ASK1/MAP3K5 Antibody (BM4220) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (BM4220) .
ASK1/MAP3K5 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-ASK1/MAP3K5 Antibody (BM4220) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ASK1/MAP3K5 using anti-ASK1/MAP3K5 antibody (BM4220) .
ASK1/MAP3K5 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-ASK1/MAP3K5 Antibody (BM4220) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Immunofluorescent analysis using the Antibody.
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