Human JAM-B/JAM2 ELISA Kit

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  Human JAM-B/JAM2 ELISA Kitstandard curve

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Human JAM-C/JAM3 ELISA Kit

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  Rat JAM-C ELISA Kit standard curve

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Human Jagged1/JAG1 ELISA Kit

Human AMICA/JAML ELISA Kit

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  Human AMICA/JAML ELISA Kit standard curve

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Mouse JAM-C/JAM3 ELISA Kit

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  Human JAM-C ELISA Kit standard curve

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Mouse JAM-C/JAM3 ELISA Kit

Human JAM-A/F11R/Junctional Adhesion Molecule 1 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Human JAM-C EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Anti-V5-tag HRP conjugated Antibody

筛选器： All WB

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  Western blot analysis of extracts from v5-tag fusion protein, using V5-tag Antibody(HRP conjugated).

Anti-Connexin 43/GJA1 Antibody

筛选器： All WB IHC ICC/IF ELISA

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  Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (A00599). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human U-87MG whole cell lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: mouse brain tissue lysates,
  Lane 4: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody (A00599) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin 43/GJA1 at approximately 43 kDa. The expected band size for Connexin 43/GJA1 is at 43 kDa.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (A00599).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (A00599) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (A00599).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (A00599) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (A00599).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (A00599) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of GJA1 using anti- GJA1 antibody (A00599).
  GJA1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-Connexin 43/GJA1 Antibody

筛选器： All WB IHC

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  Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: rat hear tissue lysates,
  Lane 4: mouse brain tissue lysates,
  Lane 5: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody (PA1026-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin 43/GJA1 at approximately 43 kDa. The expected band size for Connexin 43/GJA1 is at 43 kDa.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).
  Connexin 43/GJA1 was detected in frozen section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Anti-Connexin 43/GJA1 Antibody

筛选器： All WB IHC IF

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  Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (BA1727). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: rat brain tissue lysates,
  Lane 3: rat heart tissue lysates,
  Lane 4: mouse brain tissue lysates,
  Lane 5: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody (BA1727) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin 43/GJA1 at approximately 43 kDa. The expected band size for Connexin 43/GJA1 is at 43 kDa.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (BA1727).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (BA1727) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (BA1727).
  Connexin 43/GJA1 was detected in a paraffin-embedded section of human theart tissue. The tissue section was incubated with rabbit anti-Connexin 43/GJA1 Antibody (BA1727) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of Connexin 43/GJA1 using anti- Connexin 43/GJA1 antibody (BA1727).
  Connexin 43/GJA1 was detected in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- Connexin 43/GJA1 Antibody (BA1727) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. . Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-Connexin-26/GJB2 Antibody

筛选器： All WB FCM

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  Western blot analysis of Connexin-26/GJB2 using anti-Connexin-26/GJB2 antibody (A00512-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: PC-3 whole cell lysates,
  Lane 2: RT4 whole cell lysates,
  Lane 3: U251 whole cell lysates,
  Lane 4: rat liver tissue lysates,
  Lane 5: rat kidney tissue lysates,
  Lane 6: mouse liver tissue lysates,
  Lane 7: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-26/GJB2 antigen affinity purified polyclonal antibody (A00512-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-26/GJB2 at approximately 26 kDa. The expected band size for Connexin-26/GJB2 is at 26 kDa.

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  Flow Cytometry analysis of PC-3 cells using anti-Connexin-26/GJB2 antibody (A00512-2).
  Overlay histogram showing PC-3 cells stained with A00512-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Connexin-26/GJB2 Antibody (A00512-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of U251 cells using anti-Connexin-26/GJB2 antibody (A00512-2).
  Overlay histogram showing U251 cells stained with A00512-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Connexin-26/GJB2 Antibody (A00512-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-Connexin-26/GJB2 Antibody

筛选器： All WB ELISA

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  Western blot analysis of Connexin-26/GJB2 using anti-Connexin-26/GJB2 antibody (A00512-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: rat kidney tissue lysates,
  Lane 3: mouse liver tissue lysates,
  Lane 4: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-26/GJB2 antigen affinity purified polyclonal antibody (A00512-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-26/GJB2 at approximately 26 kDa. The expected band size for Connexin-26/GJB2 is at 26 kDa.

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Anti-Connexin-26/GJB2 Antibody

筛选器： All WB

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  Western blot analysis of Connexin-26/GJB2 using anti-Connexin-26/GJB2 antibody (BA1591-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-26/GJB2 antigen affinity purified polyclonal antibody (BA1591-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-26/GJB2 at approximately 26 kDa. The expected band size for Connexin-26/GJB2 is at 26 kDa.

Anti-Connexin-32/GJB1 Antibody

筛选器： All WB

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  Western blot analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (A01050). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat kidney tissue lysates,
  Lane 2: mouse kidney tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-32/GJB1 antigen affinity purified polyclonal antibody (A01050) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-32/GJB1 at approximately 32 kDa. The expected band size for Connexin-32/GJB1 is at 32 kDa.

Anti-Connexin-32/GJB1 Antibody

筛选器： All WB IHC

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  Western blot analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (BA1592). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Rat Cardiac Muscle tissue lysates,
  Lane 2: Rat Cardiac Muscle tissue lysates,
  Lane 3: Rat Skeletal Muscle tissue lysates,
  Lane 4: Rat Brain tissue lysates,
  Lane 5: MCF-7 whole cell lysates,
  Lane 6: HELA whole cell lysates,
  Lane 7: SMMC whole cell lysates,
  Lane 8: JURKAT whole cell lysates,
  Lane 9: COLO320 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-32/GJB1 antigen affinity purified polyclonal antibody (BA1592) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-32/GJB1 at approximately 32 kDa. The expected band size for Connexin-32/GJB1 is at 32 kDa.

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  IHC analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (BA1592).
  Connexin-32/GJB1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-Connexin-32/GJB1 Antibody (BA1592) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (BA1592).
  Connexin-32/GJB1 was detected in a paraffin-embedded section of human liver tissue. The tissue section was incubated with rabbit anti-Connexin-32/GJB1 Antibody (BA1592) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

Anti-Connexin-32/GJB1 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of anti-Connexin-32/GJB1 antibody (A01050-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-32/GJB1 antigen affinity purified polyclonal antibody (A01050-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-32/GJB1 at approximately 35 kDa. The expected band size for Connexin-32/GJB1 is at 32 kDa.

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  Flow Cytometry analysis of HepG2 cells using anti-Connexin-32/GJB1 antibody (A01050-1).
  Overlay histogram showing HepG2 cells stained with A01050-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Connexin-32/GJB1 Antibody (A01050-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Anti-Connexin-46/GJA3 Antibody

筛选器： All WB

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  Western blot analysis of Connexin-46/GJA3 using anti-Connexin-46/GJA3 antibody (PB0629). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Rat Cardiac Muscle tissue lysates,
  Lane 2: Rat Kidney tissue lysates,
  Lane 3: Human Placenta tissue lysates,
  Lane 4: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-46/GJA3 antigen affinity purified polyclonal antibody (PB0629) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-46/GJA3 at approximately 47 kDa. The expected band size for Connexin-46/GJA3 is at 47 kDa.

Anti-Connexin-46/GJA3 Antibody

筛选器： All WB

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  Western blot analysis of Connexin-46/GJA3 using anti-Connexin-46/GJA3 antibody (BA3200-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human A431 whole cell lysates,
  Lane 3: mouse NIH/3T3 whole cell lysates,
  Lane 4: human SMMC whole cell lysates,
  Lane 5: human A549 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Connexin-46/GJA3 antigen affinity purified polyclonal antibody (BA3200-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Connexin-46/GJA3 at approximately 47 kDa. The expected band size for Connexin-46/GJA3 is at 47 kDa.