Anti-BSEP/ABCB11 Antibody

筛选器： All WB IHC IF

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  Western blot analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HCCT tissue lysates,
  Lane 2: rat liver tissue lysates,
  Lane 3: mouse liver tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BSEP/ABCB11 antigen affinity purified polyclonal antibody (PB9414) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BSEP/ABCB11 at approximately 146 kDa. The expected band size for BSEP/ABCB11 is at 146 kDa.

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  IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
  BSEP/ABCB11 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
  BSEP/ABCB11 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414).
  BSEP/ABCB11 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BSEP/ABCB11 Antibody (PB9414) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of ABCB11 using anti- ABCB11 antibody (PB9414)ABCB11 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- ABCB11 Antibody (PB9414) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-RAB11A Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of RAB11A using anti-RAB11A antibody (PB0836). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human SH-SY5Y whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human MCF-7 whole cell lysates,
  Lane 5: human A549 whole cell lysates,
  Lane 6: human RT4 whole cell lysates,
  Lane 7: human Hacat whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11A antigA03957-Aen affinity purified polyclonal antibody (PB0836) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11A at approximately 24 kDa. The expected band size for RAB11A is at 24 kDa.

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  Western blot analysis of RAB11A using anti-RAB11A antibody (PB0836). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: rat lung tissue lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse spleen tissue lysates,
  Lane 7: mouse lung tissue lysates,
  Lane 8: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11A antigA03957-Aen affinity purified polyclonal antibody (PB0836) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11A at approximately 24 kDa. The expected band size for RAB11A is at 24 kDa.

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  IF analysis of RAB11A using anti-RAB11A antibody (PB0836).
  RAB11A was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-RAB11A Antibody (PB0836) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of SH-SY5Y cells using anti-RAB11A antibody (PB0836).
  Overlay histogram showing SH-SY5Y cells stained with PB0836 (Blue line). To facilitate intrRAB11Allular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB11A Antibody (PB0836) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-RAB11B Antibody

筛选器： All WB IHC FCM ICC/IF

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  Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat spleen tissue lysates,
  Lane 2: rat lung tissue lysates,
  Lane 3: rat ovary tissue lysates,
  Lane 4: rat kidney tissue lysates,
  Lane 5: mouse lung tissue lysates,
  Lane 6: mouse ovary tissue lysates,
  Lane 7: mouse kidney tissue lysates,
  Lane 8: mouse SP20 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (A04526-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.

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  Western blot analysis of RAB11B using anti-RAB11B antibody (A04526-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates,
  Lane 2: human A549 whole cell lysates,
  Lane 3: human U-87MG whole cell lysates,
  Lane 4: human PC-3 whole cell lysates,
  Lane 5: human Hela whole cell lysates,
  Lane 6: human Caco-2 whole cell lysates,
  Lane 7: human HL-60 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11B antigen affinity purified polyclonal antibody (A04526-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11B at approximately 24 kDa. The expected band size for RAB11B is at 24 kDa.

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  IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
  RAB11B was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
  RAB11B was detected in a paraffin-embedded section of human ovary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
  RAB11B was detected in a paraffin-embedded section of mouse ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of RAB11B using anti-RAB11B antibody (A04526-1).
  RAB11B was detected in a paraffin-embedded section of rat ovary tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAB11B Antibody (A04526-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of A549 cells using anti-RAB11B antibody (A04526-1).Overlay histogram showing A549 cells stained with A04526-1 (Blue line).anti-RAB11B Antibody (A04526-1, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  IF analysis of RAB11B using anti-RAB11B antibody (A04526-1).
  RAB11B was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti-RAB11B Antibody (A04526-1) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-NDUFB11 antibody

筛选器： All WB IHC ICC/IF IP ELISA

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  Western blot analysis of NDUFB11 using anti-NDUFB11 antibody (A08638-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human 293T whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: rat skeletal muscle tissue lysates,
  Lane 6: rat heart tissue lysates,
  Lane 7: mouse skeletal muscle tissue lysates,
  Lane 8: mouse heart tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NDUFB11 antigA03957-Aen affinity purified polyclonal antibody (A08638-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NDUFB11 at approximately 18 kDa. The expected band size for NDUFB11 is at 18 kDa.

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Anti-DNAJB11 Antibody

筛选器： All WB IHC ICC/IF IP ELISA

Anti-HSPB11/IFT25 Antibody

筛选器： All WB ELISA

Anti-AKR1B10 Antibody

筛选器： All WB IHC ICC/IF

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  Western blot analysis of AKR1B10 using anti-AKR1B10 antibody (A02976). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: A549 whole cell lysates,
  Lane 2: HepG2 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKR1B10 antigen affinity purified polyclonal antibody (A02976) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKR1B10 at approximately 36 kDa. The expected band size for AKR1B10 is at 36 kDa.

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  IHC analysis of AKR1B10 using anti-AKR1B10 antibody (A02976).
  AKR1B10 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AKR1B10 Antibody (A02976) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of AKR1B10 using anti-AKR1B10 antibody (A02976).
  AKR1B10 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AKR1B10 Antibody (A02976) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of AKR1B10 using anti-AKR1B10 antibody (A02976).
  AKR1B10 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AKR1B10 Antibody (A02976) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Anti-APAF1 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of APAF1 using anti-APAF1 antibody (A00889-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: HEL whole cell lysates,
  Lane 2: HL-60 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-APAF1 antigen affinity purified polyclonal antibody (A00889-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for APAF1 at approximately 142 kDa. The expected band size for APAF1 is at 142 kDa.

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  Flow Cytometry analysis of U937 cells using anti-APAF1 antibody (A00889-2).
  Overlay histogram showing U937 cells stained with A00889-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APAF1 Antibody (A00889-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-AZIN2 Antibody

筛选器： All WB IHC

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  Western blot analysis of AZIN2 using anti-AZIN2 antibody (PB1101). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: mouse brain tissue lysates,
  Lane 3: human Hela whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AZIN2 antigen affinity purified polyclonal antibody (PB1101) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AZIN2 at approximately 50 kDa. The expected band size for AZIN2 is at 50 kDa.

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  IHC analysis of AZIN2 using anti-AZIN2 antibody (PB1101).
  AZIN2 was detected in a paraffin-embedded section of human testicar cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AZIN2 Antibody (PB1101) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Anti-Alpha 1 Antitrypsin/SERPINA1 Antibody

筛选器： All WB

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  Western blot analysis of Alpha 1 Antitrypsin/SERPINA1 using anti-Alpha 1 Antitrypsin/SERPINA1 antibody (PB1151). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: human Hela whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Alpha 1 Antitrypsin/SERPINA1 antigen affinity purified polyclonal antibody (PB1151) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha 1 Antitrypsin/SERPINA1 at approximately 53 kDa. The expected band size for Alpha 1 Antitrypsin/SERPINA1 is at 47 kDa.

Anti-BCL9L Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Western blot analysis of BCL9L using anti-BCL9L antibody (A05905). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: rat testicular issue lysates, Lane 5: rat liver issue lysates, Lane 6: mouse testicular issue lysates, Lane 7: mouse thymus issue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. anti-BCL9L antigen affinity purified polyclonal antibody (Catalog # A05905)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for BCL9L at approximately 200KD. The expected band size for BCL9L is at 157KD.

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  Western blot analysis of BCL9L using anti-BCL9L antibody (A05905). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human T-47D whole cell lysates,
  Lane 3: human PANC-1 whole cell lysates,
  Lane 4: rat testicular issue lysates,
  Lane 5: rat liver issue lysates,
  Lane 6: mouse testicular issue lysates,
  Lane 7: mouse thymus issue lysates,
  Lane 8: mouse HEPA1-6 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BCL9L antigen affinity purified polyclonal antibody (A05905) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BCL9L at approximately 200 kDa. The expected band size for BCL9L is at 157 kDa.

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  IHC analysis of BCL9L using anti-BCL9L antibody (A05905).
  BCL9L was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCL9L Antibody (A05905) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BCL9L using anti-BCL9L antibody (A05905).
  BCL9L was detected in a paraffin-embedded section of human Ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCL9L Antibody (A05905) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BCL9L using anti-BCL9L antibody (A05905).
  BCL9L was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCL9L Antibody (A05905) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BCL9L using anti-BCL9L antibody (A05905).
  BCL9L was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCL9L Antibody (A05905) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BCL9L using anti-BCL9L antibody (A05905).
  BCL9L was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCL9L Antibody (A05905) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of BCL9L using anti-BCL9L antibody (A05905).
  BCL9L was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BCL9L Antibody (A05905) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of BCL9L using anti- BCL9L antibody (A05905).
  BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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  IF analysis of BCL9L using anti- BCL9L antibody (A05905).
  BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- BCL9L Antibody (A05905) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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  IF analysis of BCL9L using anti- BCL9L antibody (A05905).
  BCL9L was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- BCL9L Antibody (A05905) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-BDKRB2 Antibody

筛选器： All WB

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  Western blot analysis of BDKRB2 using anti-BDKRB2 antibody (PB1102). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human HepG2 whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human A549 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified polyclonal antibody (PB1102) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BDKRB2 at approximately 50 kDa. The expected band size for BDKRB2 is at 44 kDa.

Anti-BMP15 Antibody

筛选器： All WB

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  Western blot analysis of BMP15 using anti-BMP15 antibody (PB1103). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BMP15 antigen affinity purified polyclonal antibody (PB1103) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BMP15 at approximately 45 kDa. The expected band size for BMP15 is at 45 kDa.

Anti-CCDC6 Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of CCDC6 using anti-CCDC6 antibody (PB1106). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat testis muscle tissue lysates,
  Lane 2: human MCF-7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCDC6 antigen affinity purified polyclonal antibody (PB1106) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CCDC6 at approximately 66 kDa. The expected band size for CCDC6 is at 53 kDa.

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  IF analysis of CCDC6 using anti-CCDC6 antibody (PB1106).
  CCDC6 was detected in an immunocytochemical section of T-47D cells. The section was incubated with rabbit anti-CCDC6 Antibody (PB1106) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of Caco-2 cells using anti-CCDC6 antibody (PB1106).
  Overlay histogram showing Caco-2 cells stained with PB1106 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCDC6 Antibody (PB1106) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-CXCL16 Antibody

筛选器： All WB ELISA

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  Western blot analysis of CXCL16 using anti-CXCL16 antibody (PB1110). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human CEM whole cell lysates,
  Lane 2: human A549 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CXCL16 antigen affinity purified polyclonal antibody (PB1110) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CXCL16 at approximately 35 kDa. The expected band size for CXCL16 is at 28 kDa.

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Anti-ELA2/ELANE Antibody

筛选器： All WB IHC ELISA

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  Western blot analysis of ELA2/ELANE using anti-ELA2/ELANE antibody (PB1114). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse spleen tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ELA2/ELANE antigA03957-Aen affinity purified polyclonal antibody (PB1114) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ELA2/ELANE at approximately 29 kDa. The expected band size for ELA2/ELANE is at 29 kDa.

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  IHC analysis of ELA2/ELANE using anti-ELA2/ELANE antibody (PB1114).
  ELA2/ELANE was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-ELA2/ELANE Antibody (PB1114) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of ELA2/ELANE using anti-ELA2/ELANE antibody (PB1114).
  ELA2/ELANE was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-ELA2/ELANE Antibody (PB1114) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

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Anti-ERCC6 Antibody

筛选器： All WB

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  Western blot analysis of ERCC6 using anti-ERCC6 antibody (PB1109). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: human COLO320 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERCC6 antigen affinity purified polyclonal antibody (PB1109) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ERCC6 at approximately 168 kDa. The expected band size for ERCC6 is at 168 kDa.

Anti-FGF19 Antibody

筛选器： All WB ELISA

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  Western blot analysis of FGF19 using anti-FGF19 antibody (PB1116). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF19 antigen affinity purified polyclonal antibody (PB1116) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.

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Anti-FGF19 Antibody

筛选器： All IHC

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  IHC analysis of FGF19 using anti-FGF19 antibody (PB1117).
  FGF19 was detected in a paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-FGF19 Antibody (PB1117) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

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  IHC analysis of FGF19 using anti-FGF19 antibody (PB1117).
  FGF19 was detected in a paraffin-embedded section of rat colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-FGF19 Antibody (PB1117) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

Anti-FGF21 Antibody

筛选器： All WB ELISA

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  Western blot analysis of FGF21 using anti-FGF21 antibody (PB1118). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human placenta tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (PB1118) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF21 at approximately 22 kDa. The expected band size for FGF21 is at 22 kDa.

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  Western blot analysis of FGF21 using anti-FGF21 antibody (PB1118). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat spleen tissue lysates,
  Lane 2: rat liver tissue lysates,
  Lane 3: rat RH35 whole cell lysates,
  Lane 4: rat brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (PB1118) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF21 at approximately 22 kDa. The expected band size for FGF21 is at 22 kDa.

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  Western blot analysis of FGF21 using anti-FGF21 antibody (PB1118). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (PB1118) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGF21 at approximately 22 kDa. The expected band size for FGF21 is at 22 kDa.

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