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Western blot analysis of anti-ATPB/ATP5F1B antibody (BM5027). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATPB/ATP5F1B antigen affinity purified monoclonal antibody (BM5027) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATPB/ATP5F1B at approximately 50 kDa. The expected band size for ATPB/ATP5F1B is at 57 kDa.
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IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (BM5027) .
ATPB/ATP5F1B was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody (BM5027) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (BM5027) .
ATPB/ATP5F1B was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody (BM5027) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (BM5027) .
ATPB/ATP5F1B was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody (BM5027) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (BM5027) .
ATPB/ATP5F1B was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody (BM5027) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (BM5027) .
ATPB/ATP5F1B was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ATPB/ATP5F1B Antibody (BM5027) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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